Singh Rajkumar, Varma A J, Seeta Laxman R, Rao Mala
Biochemical Sciences Division, National Chemical Laboratory, Pune 411008, India.
Bioresour Technol. 2009 Dec;100(24):6679-81. doi: 10.1016/j.biortech.2009.07.060. Epub 2009 Aug 15.
A complete cellulase from Penicillium pinophilum was evaluated for the hydrolysis of alpha-cellulose derived from steam exploded sugarcane bagasse and other cellulosic substrates. Alpha-cellulose at 1% substrate concentration was completely hydrolyzed by Penicillium cellulase within 3h wherein at 10% the hydrolysis was 100% within 24 h with an enzyme loading of 10 FPU/g. The hydrolysate yielded glucose as major end product as analyzed by HPLC. Under similar conditions, hydrolysis of Sigmacell (microcrystalline cellulose), CP-123 (pulverized cellulose powder) and ball milled Solka Floc were 42%, 56% and 52%, respectively. Further the hydrolysis performance of Penicillium sp. cellulase is compared with Trichoderma reesei cellulase (Accellerase 1000) from Genencore. The kinetics of hydrolysis with respect to enzyme and substrate concentration will be presented.
对嗜松青霉来源的一种完整纤维素酶进行了评估，以确定其对蒸汽爆破甘蔗渣及其他纤维素底物衍生的α-纤维素的水解作用。在底物浓度为1%时，嗜松青霉纤维素酶在3小时内可将α-纤维素完全水解；在底物浓度为10%时，酶负载量为10 FPU/g，24小时内水解率达100%。经高效液相色谱分析，水解产物以葡萄糖作为主要终产物。在类似条件下，西格马纤维素（微晶纤维素）、CP - 123（粉碎的纤维素粉末）和球磨斯尔卡纤维的水解率分别为42%、56%和52%。此外，还将嗜松青霉纤维素酶的水解性能与杰能科公司的里氏木霉纤维素酶（Accellerase 1000）进行了比较。文中将给出关于酶和底物浓度的水解动力学。
