Department of Biological Science, R. J. College, Bombay, India.
Plant Cell Rep. 1991 Mar;9(11):627-30. doi: 10.1007/BF00231803.
Hairy roots of Tagetes patula were grown for 24 days in modified Murashige and Skoog's liquid medium at different initial pH levels of 4.0, 5.0, 5.7, 6.0 and 7.0. Irrespective of the initial pH, after 12 days, the pH of the culture medium was approximately 4.5. However the final pH, after 24 days of growth, did depend weakly on the initial pH of the medium. The biomass yield was lowest at an initial pH of 4.0, possibly due to lower utilization of ammonium at this pH. Similar patterns of thiophene accumulation was observed at all pH levels tested. Maximum thiophene accumulation occurred in root cultures which were 12-16 days old.
金光菊毛状根在改良的 Murashige 和 Skoog 液体培养基中生长 24 天，初始 pH 值分别为 4.0、5.0、5.7、6.0 和 7.0。无论初始 pH 值如何，培养 12 天后，培养基的 pH 值约为 4.5。然而，24 天后的最终 pH 值，弱依赖于培养基的初始 pH 值。在初始 pH 值为 4.0 时，生物量产量最低，这可能是由于在此 pH 值下铵的利用率较低。在所有测试的 pH 值水平下，噻吩的积累模式都相似。噻吩的最大积累发生在 12-16 天大的根培养物中。
