Lee J H, Pagan R J, Rogers P L
School of Biotechnology, University of New South Wales, Sydney, NSW 2033, Australia.
Biotechnol Bioeng. 1983 Mar;25(3):659-69. doi: 10.1002/bit.260250304.
A continuous process involving simultaneous saccharification and fermentation of liquefied starch has been developed using Zymomonas mobilis. Amyloglucosidase retention and cell recycle have been effected by using an Amicon hollow-fiber membrane system with a MW cutoff of 5000. Relatively high productivities of up to 60 g L(-1) h(-1) have been achieved at ethanol concentrations of 60-65 g/L. The system also offers the potential for reduced enzyme requirements for saccharification.
利用运动发酵单胞菌开发了一种连续过程,该过程涉及液化淀粉的同步糖化和发酵。通过使用截留分子量为5000的Amicon中空纤维膜系统实现了淀粉葡萄糖苷酶的保留和细胞循环。在乙醇浓度为60-65 g/L时,实现了高达60 g L(-1) h(-1)的相对较高生产率。该系统还具有降低糖化所需酶量的潜力。