Laboratories of Bioprocess Development, Federal University of Rio de Janeiro-Center of Technology-School of Chemistry, 21949-900 Rio de Janeiro, Brazil.
Appl Biochem Biotechnol. 2010 May;161(1-8):93-105. doi: 10.1007/s12010-009-8810-x. Epub 2009 Oct 30.
Considerable efforts have been made to utilize agricultural and forest residues as biomass feedstock for the production of second-generation bioethanol as an alternative fuel. Fermentation utilizing strains of Zymomonas mobilis and the use of simultaneous saccharification and fermentation (SSF) process has been proposed. Statistical experimental design was used to optimize the conditions of SSF, evaluating solid content, enzymatic load, and cell concentration. The optimum conditions were found to be solid content (30%), enzymatic load (25 filter paper units/g), and cell concentration (4 g/L), resulting in a maximum ethanol concentration of 60 g/L and a volumetric productivity of 1.5 g L(-1) h(-1).
人们已经做出了相当大的努力，利用农业和林业残余物作为生物质原料，生产第二代生物乙醇作为替代燃料。利用运动发酵单胞菌菌株和同时糖化和发酵（SSF）工艺进行发酵已经被提出。统计实验设计用于优化 SSF 的条件，评估固体含量、酶负荷和细胞浓度。发现最佳条件为固体含量（30%）、酶负荷（25 滤纸单位/g）和细胞浓度（4 g/L），从而得到最大乙醇浓度为 60 g/L 和体积产率为 1.5 g L(-1) h(-1)。
