Liu Xiao-Ning, Yin Qi, Zhang Hao, Zhang Hong, Zhu Shen-Jun, Wei Ying-Jie, Hu Sheng-Shou
Key Laboratory of Cardiovascular Regenerative Medicine, Ministry of Health, and Department of Cardiovascular Surgery, Cardiovascular Institute of Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China.
Biomed Environ Sci. 2008 Apr;21(2):110-7. doi: 10.1016/S0895-3988(08)60015-X.
To investigate whether cardiac tissue extracts from rats could mimic the cardiac microenvironment and act as a natural inducer in promoting the differentiation of bone marrow stromal cells (BMSCs) into cardiomyocytes.
Three kinds of tissue extract or cell lysate [infarcted myocardial tissue extract (IMTE), normal myocardial tissue extract (NMTE) and cultured neonatal myocardial lysate (NML)] were employed to induce BMSCs into cardiomyocyte-like cells. The cells were harvested at each time point for reverse transcription-polymerase chain reaction (RT-PCR) detection, immunocytochemical analysis, and transmission electron microscopy.
After a 7-day induction, BMSCs were enlarged and polygonal in morphology. Myofilaments, striated sarcomeres, Z-lines, and more mitochondia were observed under transmission electron microscope. Elevated expression levels of cardiac-specific genes and proteins were also confirmed by RT-PCR and immunocytochemistry. Moreover, IMTE showed a greater capacity of differentiating BMSCs into cardiomyocyte-like cells.
Cardiac tissue extracts, especially IMTE, can effectively differentiate BMSCs into cardiomyocyte-like cells.
研究大鼠心脏组织提取物是否能够模拟心脏微环境，并作为一种天然诱导剂促进骨髓基质细胞（BMSCs）向心肌细胞分化。
使用三种组织提取物或细胞裂解液[梗死心肌组织提取物（IMTE）、正常心肌组织提取物（NMTE）和培养的新生心肌裂解液（NML）]将BMSCs诱导分化为类心肌细胞。在每个时间点收获细胞，进行逆转录聚合酶链反应（RT-PCR）检测、免疫细胞化学分析和透射电子显微镜检查。
诱导7天后，BMSCs形态变大且呈多边形。透射电子显微镜下观察到肌丝、横纹肌小节、Z线和更多的线粒体。RT-PCR和免疫细胞化学也证实了心脏特异性基因和蛋白质表达水平升高。此外，IMTE将BMSCs分化为类心肌细胞的能力更强。
心脏组织提取物，尤其是IMTE，能够有效地将BMSCs分化为类心肌细胞。
