大鼠梗死心肌组织提取物对促进骨髓基质细胞分化为心肌样细胞的作用。
Tissue extracts from infarcted myocardium of rats in promoting the differentiation of bone marrow stromal cells into cardiomyocyte-like cells.
作者信息
Liu Xiao-Ning, Yin Qi, Zhang Hao, Zhang Hong, Zhu Shen-Jun, Wei Ying-Jie, Hu Sheng-Shou
机构信息
Key Laboratory of Cardiovascular Regenerative Medicine, Ministry of Health, and Department of Cardiovascular Surgery, Cardiovascular Institute of Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China.
出版信息
Biomed Environ Sci. 2008 Apr;21(2):110-7. doi: 10.1016/S0895-3988(08)60015-X.
OBJECTIVE
To investigate whether cardiac tissue extracts from rats could mimic the cardiac microenvironment and act as a natural inducer in promoting the differentiation of bone marrow stromal cells (BMSCs) into cardiomyocytes.
METHODS
Three kinds of tissue extract or cell lysate [infarcted myocardial tissue extract (IMTE), normal myocardial tissue extract (NMTE) and cultured neonatal myocardial lysate (NML)] were employed to induce BMSCs into cardiomyocyte-like cells. The cells were harvested at each time point for reverse transcription-polymerase chain reaction (RT-PCR) detection, immunocytochemical analysis, and transmission electron microscopy.
RESULTS
After a 7-day induction, BMSCs were enlarged and polygonal in morphology. Myofilaments, striated sarcomeres, Z-lines, and more mitochondia were observed under transmission electron microscope. Elevated expression levels of cardiac-specific genes and proteins were also confirmed by RT-PCR and immunocytochemistry. Moreover, IMTE showed a greater capacity of differentiating BMSCs into cardiomyocyte-like cells.
CONCLUSIONS
Cardiac tissue extracts, especially IMTE, can effectively differentiate BMSCs into cardiomyocyte-like cells.
目的
研究大鼠心脏组织提取物是否能够模拟心脏微环境，并作为一种天然诱导剂促进骨髓基质细胞（BMSCs）向心肌细胞分化。
方法
使用三种组织提取物或细胞裂解液[梗死心肌组织提取物（IMTE）、正常心肌组织提取物（NMTE）和培养的新生心肌裂解液（NML）]将BMSCs诱导分化为类心肌细胞。在每个时间点收获细胞，进行逆转录聚合酶链反应（RT-PCR）检测、免疫细胞化学分析和透射电子显微镜检查。
结果
诱导7天后，BMSCs形态变大且呈多边形。透射电子显微镜下观察到肌丝、横纹肌小节、Z线和更多的线粒体。RT-PCR和免疫细胞化学也证实了心脏特异性基因和蛋白质表达水平升高。此外，IMTE将BMSCs分化为类心肌细胞的能力更强。
结论
心脏组织提取物，尤其是IMTE，能够有效地将BMSCs分化为类心肌细胞。
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