Su Yu-Qing, Yu Qiong, Liu Xu, Liang Yan-Lian, Wei Tian-Li
Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Jun;16(3):691-3.
In order to study the polymorphism of Landsteiner-Wiener (LW) blood group gene in Chinese population, peripheral blood samples anticoagulated with EDTA from 160 unrelated volunteer blood donors were randomly collected, and genomic DNA were extracted. 160 DNA samples were analyzed for exon 1 of LW gene by direct DNA sequencing, and detected for LWa/LWb allele by improved PCR-SSP genotyping. The results showed that all LW allele in 160 donors were LWa homozygous, and the LWa allele occurred commonly. In conclusion, LWa allele occurs with incidence of 100% of donors in this study, while LWb allele has not been found in Chinese population.
为研究中国人群中兰斯泰纳-维纳（LW）血型基因的多态性，随机采集了160名无亲缘关系的无偿献血者用乙二胺四乙酸（EDTA）抗凝的外周血样本，并提取了基因组DNA。采用直接DNA测序法对160份DNA样本的LW基因第1外显子进行分析，采用改良聚合酶链反应-序列特异性引物（PCR-SSP）基因分型法检测LWa/LWb等位基因。结果显示，160名献血者的所有LW等位基因均为LWa纯合子，LWa等位基因普遍存在。综上所述，本研究中LWa等位基因在献血者中的发生率为100%，而在中国人群中未发现LWb等位基因。
