Bigot Aurélie, Doyen Périne, Vasseur Paule, Rodius François
Lab. I.E.B.E-CNRS UMR 7146, Université de Metz, Rue Delestraint, 57070 Metz, France.
Ecotoxicol Environ Saf. 2009 Feb;72(2):382-7. doi: 10.1016/j.ecoenv.2008.04.014. Epub 2008 Jun 11.
The aim of this study was to identify a metallothionein (MT) coding sequence from the freshwater bivalve Corbicula fluminea and to measure the seasonal transcriptional pattern of MT in parallel with several detoxification genes: superoxide dismutase (SOD), catalase (CAT), glutathione S-transferases (GST) and glutathione peroxidases (GPx), in the digestive gland and the gills of this bivalve during a 1-year period. We identified a C. fluminea MT complete cDNA sequence using RT-PCR and RACE-PCR. The amino acid sequence deduced from the coding sequence encodes for a protein of 73 amino acids containing 21 cysteine residues. This protein exhibits high identities and similarities with the MT sequences of numerous bivalves. MT, SOD, CAT, pi-GST and Se-GPx expression patterns did not exhibit major seasonal variations. A slight increase of MT was observed in July. Therefore, the mRNA expression of these five genes could be used as biomarkers for monitoring studies.
本研究的目的是从淡水双壳贝类河蚬中鉴定金属硫蛋白（MT）编码序列，并在一年时间内，并行测量该双壳贝类消化腺和鳃中MT与几种解毒基因（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GST）和谷胱甘肽过氧化物酶（GPx））的季节性转录模式。我们使用逆转录聚合酶链反应（RT-PCR）和快速扩增cDNA末端（RACE-PCR）鉴定了河蚬MT完整的cDNA序列。从编码序列推导的氨基酸序列编码一个含73个氨基酸且有21个半胱氨酸残基的蛋白质。该蛋白质与众多双壳贝类的MT序列具有高度的一致性和相似性。MT、SOD、CAT、pi-GST和Se-GPx的表达模式未表现出明显的季节性变化。7月观察到MT略有增加。因此，这五个基因的mRNA表达可作为监测研究的生物标志物。
