Laboratoire des Interactions Ecotoxicologie, Biodiversité, Ecosystèmes, Université Paul Verlaine-Metz, CNRS UMR 7146, Rue Delestraint, 57070 Metz, France.
Environ Toxicol. 2011 Nov;26(6):623-32. doi: 10.1002/tox.20599. Epub 2010 Apr 12.
The aim of the present study was to measure the early effects of copper (10 and 50 μg L(-1)), cadmium (2, 10, and 50 μg L(-1)) and mixtures of these metals in the freshwater bivalve Corbicula fluminea exposed for 12 h in laboratory. Transcription levels of superoxide dismutase (SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GPx), pi-class glutathione S-transferase (pi-GST), metallothionein (MT) in digestive gland and gills, and response of lysosomal system and neutral lipids in digestive gland were determined after the exposure period. Results showed that lysosomal system, neutral lipids content, and mRNA levels were modified, suggesting their early response against oxidative stress and their important role in cell integrity. The integrated biomarker response was calculated and showed that the effects of the combinations of Cu and Cd on the biomarker responses are additive. MT and pi-GST mRNA expression correspond to the largest ranges of response. As efficient biomarkers should have an early warning capacity, SOD, CAT, Se-GPx, pi-GST, MT transcripts levels, lysosomal system, and neutral lipids could be used as biomarkers of metal contamination in the aquatic environment.
本研究旨在测量铜（10 和 50μg/L）、镉（2、10 和 50μg/L）以及这些金属混合物在淡水双壳类贻贝 Corbicula fluminea 中的早期影响，贻贝在实验室中暴露 12 小时。在暴露期后，测定了消化腺和鳃中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、硒依赖性谷胱甘肽过氧化物酶（Se-GPx）、π 类谷胱甘肽 S-转移酶（pi-GST）、金属硫蛋白（MT）的转录水平，以及消化腺中溶酶体系统和中性脂质的反应。结果表明，溶酶体系统、中性脂质含量和 mRNA 水平发生了变化，表明它们对氧化应激的早期反应及其在细胞完整性中的重要作用。计算了综合生物标志物反应，结果表明 Cu 和 Cd 组合对生物标志物反应的影响是相加的。MT 和 pi-GST mRNA 表达对应于最大的反应范围。由于有效的生物标志物应该具有早期预警能力，因此 SOD、CAT、Se-GPx、pi-GST、MT 转录水平、溶酶体系统和中性脂质可作为水生环境中金属污染的生物标志物。
