Rostkowska-Nadolska Beata, Kuśmierz Dariusz, Kapral Małgorzata, Latocha Małgorzata, Swiatkowska Leongina, Fraczek Marcin
Katedra i Klinika Otolaryngologii Akademii Medycznej we Wrocławiu.
Otolaryngol Pol. 2007;61(5):661-7. doi: 10.1016/S0030-6657(07)70503-6.
Recurrent polyposis in the same patient resulting in the necessity of repeated surgeries forced to search for new pharmacological therapeutic methods. At present, locally acting glycocorticosteroids have the greatest value in the treatment of nasal polyposis. Polyps grow is connected with inflammation process and proliferation of fibroblasts.
An evaluation of calcitriol and tacalcitol influence on proliferation of fibroblasts extracted from nasal polyps.
consisted of 9 tissue samples coming from nasal polyps sampled during polypectomies. The testing was performed on the polyps fibroblasts after the sixth passage after the primary culture was established. Three days after the culture was started the cells were poured with nutrient medium without serum added and after further 24 hours was replaced by nutrient medium with takalcitol and calcitriol in the defined concentrations. The expression of the genes coding histone H3 was evaluated with the use of RT-PCR technique.
Tacalcitiol and calcitriol in vitro decrease proliferation of fibroblasts sampled from nasal polyps. Inhibition is most effective for the concentration of 10-4M. Tacalcitiol and calcitriol also inhibit level of histone H3 gene expression.
Experimental data suggest tacalcitiol to be more effective in the same concentration. Present studies may indicate the direction of further investigation in the potential pharmacological treatment on nasal polyps.
同一患者反复出现息肉，导致需要重复手术，这促使人们寻找新的药物治疗方法。目前，局部作用的糖皮质激素在鼻息肉治疗中具有最大价值。息肉生长与炎症过程和成纤维细胞增殖有关。
评估骨化三醇和他卡西醇对从鼻息肉中提取的成纤维细胞增殖的影响。
由9个在息肉切除术中采集的鼻息肉组织样本组成。在原代培养建立后的第六代对息肉成纤维细胞进行测试。培养开始三天后，向细胞中加入无血清的营养培养基，再过24小时后，用含有确定浓度的他卡西醇和骨化三醇的营养培养基替换。使用RT-PCR技术评估编码组蛋白H3的基因表达。
他卡西醇和骨化三醇在体外可降低从鼻息肉中提取的成纤维细胞的增殖。对于10-4M的浓度，抑制作用最为有效。他卡西醇和骨化三醇还抑制组蛋白H3基因的表达水平。
实验数据表明，在相同浓度下他卡西醇更有效。目前的研究可能为鼻息肉潜在药物治疗的进一步研究指明方向。
