Department of Otolaryngology, Wroclaw Medical University, Wroclaw, Poland.
Adv Med Sci. 2010;55(1):86-92. doi: 10.2478/v10039-010-0012-9.
Biologically active vitamin D3 (VD) derivatives possess modulatory activities on immunological and inflammatory responses which can be reflected by altered levels of pro-inflammatory chemokines. Nasal polyposis (NP), defined as a chronic inflammatory process of upper respiratory system, could be influenced by VD derivatives. The purpose of this study was to investigate the influence of 1alpha,25-dihydroxyvitamin D3 (calcitriol) and 1alpha,24(R)-dihydroxyvitamin D3 (tacalcitol) on the secretion of IL-6 and IL-8 by fibroblasts derived from NP.
The study involved 12 fibroblast cultures derived from NP samples obtained from surgically treated patients. Measurements were performed on the polyp cells after the 6-9 passages. Culture stimulation involved treatment with tacalcitol and calcitriol at a defined strength (from 10(-7)M to 10(-4)M). IL-6 and IL-8 concentrations were estimated with ELISA.
Treatment with calcitriol or tacalcitol inhibits the synthesis of both IL-6 and IL-8 compared to the control group. The dose dependence of this effect has been confirmed. VD derivatives influence was marked at higher concentrations. Significant interleukin decrease was observed at 10(-5) and 10(-4) for calcitriol and 10-4 in the case of tacalcitol.
The present study demonstrates that calcitriol and tacalcitol are capable of affecting pro-inflammatory cytokine (IL-6 and IL-8) levels in NP cultures. Our data imply a potential therapeutical application of topical VD derivates in NP and warrant further investigation.
生物活性维生素 D3（VD）衍生物具有调节免疫和炎症反应的功能，这可以通过改变促炎趋化因子的水平来反映。鼻息肉（NP）被定义为上呼吸道系统的慢性炎症过程，可能受到 VD 衍生物的影响。本研究的目的是研究 1α，25-二羟维生素 D3（骨化三醇）和 1α，24（R）-二羟维生素 D3（他卡西醇）对 NP 来源的成纤维细胞分泌 IL-6 和 IL-8 的影响。
该研究涉及从接受手术治疗的患者中获得的 NP 样本中获得的 12 个成纤维细胞培养物。在第 6-9 次传代后对息肉细胞进行测量。使用 tacalcitol 和 calcitriol 进行培养刺激，浓度为 10（-7）M 至 10（-4）M。使用 ELISA 测定 IL-6 和 IL-8 浓度。
与对照组相比，calcitriol 或 tacalcitol 的处理抑制了两种 IL-6 和 IL-8 的合成。已经证实了这种作用的剂量依赖性。VD 衍生物的影响在较高浓度时更为明显。与对照组相比，calcitriol 在 10（-5）和 10（-4）时，tacalcitol 在 10-4 时观察到显著的白细胞介素下降。
本研究表明，骨化三醇和他卡西醇能够影响 NP 培养物中促炎细胞因子（IL-6 和 IL-8）的水平。我们的数据表明局部 VD 衍生物在 NP 中的潜在治疗应用，并需要进一步研究。
