Alberti Kristin, Davey Ryan E, Onishi Kento, George Sophia, Salchert Katrin, Seib F Philipp, Bornhäuser Martin, Pompe Tilo, Nagy Andras, Werner Carsten, Zandstra Peter W
Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Hohe Str. 6, D-01069 Dresden, Germany.
Nat Methods. 2008 Jul;5(7):645-50. doi: 10.1038/nmeth.1222. Epub 2008 Jun 15.
The mode of ligand presentation has a fundamental role in organizing cell fate throughout development. We report a rapid and simple approach for immobilizing signaling ligands to maleic anhydride copolymer thin-film coatings, enabling stable signaling ligand presentation at interfaces at defined concentrations. We demonstrate the utility of this platform technology using leukemia inhibitory factor (LIF) and stem cell factor (SCF). Immobilized LIF supported mouse embryonic stem cell (mESC) pluripotency for at least 2 weeks in the absence of added diffusible LIF. Immobilized LIF activated signal transducer and activator of transcription 3 (STAT3) and mitogen-activated protein kinase (MAPK) signaling in a dose-dependent manner. The introduced method allows for the robust investigation of cell fate responses from interface-immobilized ligands.
配体呈现方式在整个发育过程中组织细胞命运方面起着基本作用。我们报告了一种快速且简单的方法，可将信号配体固定在马来酸酐共聚物薄膜涂层上，从而在界面处以确定的浓度实现信号配体的稳定呈现。我们使用白血病抑制因子（LIF）和干细胞因子（SCF）证明了该平台技术的实用性。固定化的LIF在不添加可扩散LIF的情况下，支持小鼠胚胎干细胞（mESC）多能性至少2周。固定化的LIF以剂量依赖的方式激活信号转导子和转录激活子3（STAT3）以及丝裂原活化蛋白激酶（MAPK）信号传导。所引入的方法能够对来自界面固定化配体的细胞命运反应进行深入研究。
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