Park Y, Davis M E, Wallis D A
Department of Chemical Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-6496.
Biotechnol Bioeng. 1984 May;26(5):468-76. doi: 10.1002/bit.260260510.
The dynamic analysis of a continuous, aerobic, fixed-film bioreactor has been performed. Rigorous mathematical models have been developed for a fluidized-bed fermentor with biofilm growth. The transient performance of the reactor is appraised in terms of outlet penicillin concentration for constant, as well as variable carbon substrate feed rates. The effect of the reactor oxygen transfer capacity is elucidated for those cases employing substrate feeding strategies. The results show that penicillin production in a continuous, fixed-film bioreactor reaches a maximum with processing time, but subsequently decreases as cell mass accumulates and substrate deficiencies occur. The maximum production level can be maintained for increased operating times if the substrate supply is continuously increased. The duration of this prolonged production is a direct function of the rate of increase and the operating time at which the increase is initiated. The oxygen transfer capacity of the reactor was found to be important to the effectiveness of a feeding strategy.
已对连续式好氧固定膜生物反应器进行了动力学分析。针对具有生物膜生长的流化床发酵罐开发了严格的数学模型。根据恒定以及可变碳底物进料速率下的出口青霉素浓度来评估反应器的瞬态性能。对于采用底物进料策略的情况，阐明了反应器氧气传递能力的影响。结果表明，连续式固定膜生物反应器中的青霉素产量随处理时间达到最大值，但随后随着细胞质量积累和底物不足而下降。如果持续增加底物供应，则可以在延长的操作时间内维持最大生产水平。这种延长生产的持续时间是增加速率和开始增加时的操作时间的直接函数。发现反应器的氧气传递能力对进料策略的有效性很重要。
