Park Y, Davis M E, Wallis D A
Department of Chemical Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061.
Biotechnol Bioeng. 1984 May;26(5):457-67. doi: 10.1002/bit.260260509.
The analysis of a continuous, aerobic, fixed-film bioreactor is performed by simulating the behavior of penicillin production in a three-phase fluidized bed. Rigorous mathematical models are developed for a fluidized-bed fermentor in which bioparticles are fluidized by the liquid medium and air. The steady-state performance of the fluidized-bed reactor is appraised in terms of penicillin productivity and outlet concentration by considering the two extremes in contacting patterns, complete back-mix and plug flow, in the absence of a growing biofilm. The results show that the complete back-mix contacting pattern is preferred over that of plug flow due to the nature of the penicillin kinetic relationships. It is also shown that for the dual-nutrient (glucose and oxygen) penicillin reaction system the optimum biofilm thickness does not equal the penetration depth of a limiting nutrient, but depends upon the total reactor configuration.
通过模拟青霉素在三相流化床中的生产行为，对连续、好氧、固定膜生物反应器进行了分析。针对流化床发酵罐建立了严格的数学模型，在该发酵罐中生物颗粒由液体介质和空气流化。在不存在生长生物膜的情况下，通过考虑两种极端的接触模式，即完全返混和活塞流，从青霉素生产率和出口浓度方面评估了流化床反应器的稳态性能。结果表明，由于青霉素动力学关系的性质，完全返混接触模式优于活塞流接触模式。还表明，对于双营养物（葡萄糖和氧气）青霉素反应系统，最佳生物膜厚度不等于限制营养物的穿透深度，而是取决于整个反应器的配置。
