Sugiura Shinji, Edahiro Jun-ichi, Kikuchi Kyoko, Sumaru Kimio, Kanamori Toshiyuki
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology, Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Biotechnol Bioeng. 2008 Aug 15;100(6):1156-65. doi: 10.1002/bit.21836.
This article reports a pressure-driven perfusion culture chip developed for parallel drug cytotoxicity assay. The device is composed of an 8 x 5 array of cell culture microchambers with independent perfusion microchannels. It is equipped with a simple interface for convenient access by a micropipette and connection to an external pressure source, which enables easy operation without special training. The unique microchamber structure was carefully designed with consideration of hydrodynamic parameters and was fabricated out of a polydimethylsiloxane by using multilayer photolithography and replica molding. The microchamber structure enables uniform cell loading and perfusion culture without cross-contamination between neighboring microchambers. A parallel cytotoxicity assay was successfully carried out in the 8 x 5 microchamber array to analyze the cytotoxic effects of seven anticancer drugs. The pressure-driven perfusion culture chip, with its simple interface and well-designed microfluidic network, will likely become an advantageous platform for future high-throughput drug screening by microchip.
本文报道了一种为并行药物细胞毒性测定而开发的压力驱动灌注培养芯片。该装置由一个8×5阵列的细胞培养微腔组成，带有独立的灌注微通道。它配备了一个简单的接口，便于微量移液器操作并连接到外部压力源，无需特殊培训即可轻松操作。独特的微腔结构在设计时仔细考虑了流体动力学参数，并通过多层光刻和复制模塑由聚二甲基硅氧烷制成。这种微腔结构能够实现均匀的细胞接种和灌注培养，相邻微腔之间不会发生交叉污染。在8×5微腔阵列中成功进行了并行细胞毒性测定，以分析七种抗癌药物的细胞毒性作用。这种压力驱动灌注培养芯片，凭借其简单的接口和精心设计的微流体网络，有望成为未来通过微芯片进行高通量药物筛选的有利平台。
