Liu Shuai, Zhang Yuanyuan, Liu Yuejian, Qin Huamin, Wang Xiaoqi, Yan Qiu
Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian, People's Republic of China.
IUBMB Life. 2008 Jul;60(7):461-6. doi: 10.1002/iub.62.
Implantation is a complex developmental event that is initiated by recognition and adhesion of the embryo to the endometrial epithelium. sLeX is an oligosaccharide antigen acting as the ligand of L-selectin, and is stage-specifically expressed in the endometrial epithelium. The adhesion system mediated by L-selectin and sLeX oligosaccharide plays an important role in this process. FUT7 is a key enzyme for sLeX synthesis, and the regulation of sLeX through FUT7 may influence maternal-fetal recognition. In this study, we observed the effect of FUT7 antisense oligodeoxynucleotide on the expression of FUT7 and sLeX, as well as adhesion in an in vitro implantation model consisting of the human uterine epithelial cell line RL95-2 and the human embryonic cell line JAR. Results showed that the expression of FUT7 was significantly decreased, compared with controls, after FUT7 antisense oligodeoxynucleotide transfection into RL95-2 cells, as determined by RT-PCR, Western blotting, and indirect immunofluorescence. Synthesis of sLeX was also decreased, consistent with the FUT7 decrease, as shown by indirect immunofluorescence. The adhesion of embryonic cells to uterine epithelial cells was significantly reduced (P < 0.01) compared with the control. These data indicate that the use of a FUT7 antisense oligodeoxynucleotide can cause a significant reduction of both FUT7 and sLeX antigen, and thereby inhibit the adhesion of embryo cells to endometrium. This approach may provide a new way to regulate reproduction.
着床是一个复杂的发育事件，由胚胎与子宫内膜上皮的识别和黏附引发。唾液酸化路易斯寡糖X（sLeX）是一种作为L-选择素配体的寡糖抗原，在子宫内膜上皮中呈阶段特异性表达。由L-选择素和sLeX寡糖介导的黏附系统在此过程中起重要作用。岩藻糖基转移酶7（FUT7）是sLeX合成的关键酶，通过FUT7对sLeX的调控可能影响母胎识别。在本研究中，我们在由人子宫上皮细胞系RL95-2和人胚胎细胞系JAR组成的体外着床模型中，观察了FUT7反义寡脱氧核苷酸对FUT7和sLeX表达以及黏附的影响。结果显示，通过逆转录-聚合酶链反应（RT-PCR）、蛋白质免疫印迹法和间接免疫荧光法测定，将FUT7反义寡脱氧核苷酸转染到RL95-2细胞后，与对照组相比，FUT7的表达显著降低。如间接免疫荧光所示，sLeX的合成也减少，与FUT7的减少一致。与对照组相比，胚胎细胞与子宫上皮细胞的黏附显著降低（P<0.01）。这些数据表明，使用FUT7反义寡脱氧核苷酸可导致FUT7和sLeX抗原显著减少，从而抑制胚胎细胞与子宫内膜的黏附。这种方法可能为调节生殖提供一种新途径。
