Shaeri Jobin, Wright Ian, Rathbone Elner B, Wohlgemuth Roland, Woodley John M
Department of Biochemical Engineering, University College London, Torrington Place, London, UK.
Biotechnol Bioeng. 2008 Nov 1;101(4):761-7. doi: 10.1002/bit.21949.
In this article we report on the characterization of the enzymatic synthesis of D-xylulose 5-phosphate using triosephosphate isomerase and transketolase. Two potential starting substrates are possible with this scheme. The data presented here allow a comparison of both routes for the synthesis, based on experimental information on reaction kinetics. Operational guidelines are proposed which should assist in the scale-up of such syntheses.
在本文中，我们报告了利用磷酸丙糖异构酶和转酮醇酶对5-磷酸-D-木酮糖进行酶促合成的特性。此方案有两种潜在的起始底物。本文所呈现的数据基于反应动力学的实验信息，对两种合成途径进行了比较。提出了操作指南，应有助于此类合成的放大。
