School of Biological Sciences, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK.
FEBS Lett. 2013 Nov 1;587(21):3422-7. doi: 10.1016/j.febslet.2013.09.022. Epub 2013 Sep 23.
The glycolytic enzyme triose phosphate isomerase from Schistosoma mansoni is a potential target for drugs and vaccines. Molecular modelling of the enzyme predicted that a Ser-Ala-Asp motif which is believed to be a helminth-specific epitope is exposed. The enzyme is dimeric (as judged by gel filtration and cross-linking), resistant to proteolysis and highly stable to thermal denaturation (melting temperature of 82.0 °C). The steady-state kinetic parameters are high (Km for dihydroxyacetone phosphate is 0.51 mM; Km for glyceraldehyde 3-phosphate is 1.1 mM; kcat for dihydroxyacetone phosphate is 7800 s(-1) and kcat for glyceraldehyde 3-phosphate is 6.9s(-1)).
曼氏血吸虫糖酵解酶磷酸丙糖异构酶是药物和疫苗的潜在靶点。对该酶的分子建模预测，一个丝氨酸-丙氨酸-天冬氨酸基序（被认为是一种寄生虫特异性表位）暴露在外。该酶是二聚体（通过凝胶过滤和交联判断），具有抗蛋白水解和对热变性（熔点为 82.0°C）高度稳定的特性。稳态动力学参数较高（二羟丙酮磷酸的 Km 为 0.51mM；甘油醛 3-磷酸的 Km 为 1.1mM；二羟丙酮磷酸的 kcat 为 7800s(-1)，甘油醛 3-磷酸的 kcat 为 6.9s(-1)）。
