Fanta G F, Abbott T P, Herman A I, Burr R C, Doane W M
Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604.
Biotechnol Bioeng. 1984 Sep;26(9):1122-5. doi: 10.1002/bit.260260916.
Treatment of wheat straw with 1N trifluoroacetic acid (TFA) for 7 h at reflux temperature yielded 23% xylose based upon initial straw weight. This corresponds to about an 80% xylose yield based on the xylan content of the hemicellulose. The cellulose component of wheat straw was largely unaffected, as evidenced by low glucose yields. Decomposition of xylose by prolonged refluxing (23 h) was minimal in 1N TFA compared to 1N HCl. Treatment of wheat straw with refluxing 1N TFA converts about 10% of the lignin initially present in straw into water-soluble lignin fragments. Fermentation of the xylose-rich wheat straw hydrolyzate to ethanol with Pachysolen tannophilus was comparable to the fermentation of reagent grade xylose, indicating that furfural and toxic lignin by-products were not produced by 1N TFA in sufficient amounts to impair cell growth and ethanol production. Cellulase treatment of the wheat straw residue after TFA hydrolysis resulted in a 70-75% conversion of the cellulose into glucose.
将麦秸与1N三氟乙酸（TFA）在回流温度下处理7小时，基于初始麦秸重量，木糖产率为23%。这相当于基于半纤维素中木聚糖含量的木糖产率约为80%。麦秸的纤维素成分基本未受影响，葡萄糖产率低就证明了这一点。与1N盐酸相比，在1N TFA中长时间回流（23小时）导致的木糖分解极少。用回流的1N TFA处理麦秸会使麦秸中最初存在的约10%的木质素转化为水溶性木质素片段。用嗜鞣管囊酵母将富含木糖的麦秸水解产物发酵为乙醇，其效果与试剂级木糖的发酵效果相当，这表明1N TFA不会产生足以损害细胞生长和乙醇生产的糠醛和有毒木质素副产物。TFA水解后对麦秸残渣进行纤维素酶处理，可使70 - 75%的纤维素转化为葡萄糖。
