National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Bioresour Technol. 2010 Jul;101(13):4875-83. doi: 10.1016/j.biortech.2010.01.063. Epub 2010 Feb 13.
Pretreating wheat straw (WS) with combined use of varied sulfuric acid concentration (0-3%, w/v) and Tween 20 concentration (0-1%) was investigated in an attempt to enhance the hydrolysis and fermentability of pretreated WS. Enzymatic hydrolysis yield of glucan and xylan and ethanol production by simultaneous saccharification and fermentation (SSF) of water-insoluble solids (WIS) were significantly affected by the amount of Tween 20 added during acid pretreatment. Any further addition of Tween 20 in either hydrolysis stage or fermentation stage only led to small increase in glucan conversion and ethanol production. Determination of adsorption of cellulases during hydrolysis showed that Tween 20-assisted acid treated straw solution contained more free cellulases than individual acid treated straw solution, indicating that modification of lignin surface by Tween 20 added during pretreatment likely occurred. In addition, the effects of pretreatment conditions on overall recovery of glucose and xylose after pretreatment and enzymatic hydrolysis were also investigated.
预处理小麦秸秆（WS）时联合使用不同硫酸浓度（0-3%，w/v）和吐温 20 浓度（0-1%），以提高预处理 WS 的水解和发酵性能。酶解纤维素和木聚糖的得率以及水不溶性固体（WIS）同步糖化发酵（SSF）生产乙醇的能力，受到酸预处理过程中添加的吐温 20 量的显著影响。在水解阶段或发酵阶段进一步添加吐温 20 仅导致纤维素转化率和乙醇产量略有增加。在水解过程中测定纤维素酶的吸附情况表明，吐温 20 辅助酸处理的秸秆溶液比单独酸处理的秸秆溶液含有更多的游离纤维素酶，表明预处理过程中添加的吐温 20 可能对木质素表面进行了修饰。此外，还研究了预处理条件对预处理和酶解后葡萄糖和木糖总回收率的影响。
