Molin G, Nilsson I
Swedish Meat Research Institute, P.O. Box 504, S-244 00 Kävlinge, Sweden.
Biotechnol Bioeng. 1985 Jan;27(1):117-20. doi: 10.1002/bit.260270114.
Pseudomonas putida ATCC 11172 was grown in chemostat on L-asparagine or phenol as the sole, limiting carbon and energy source. The growth characteristics of a culture where a biofilm was present, were compared with one where the biofilm was strongly reduced by the grinding and shearing effect of sand suspended in the culture. In the presence of the intact biofilm, the curve of steady-state biomass versus dilution rate diverged greatly from the theoretical pattern predicted by conventional chemostat models. The sand strongly retarded the biofilm formation and to a high degree restored the shape of the biomass versus dilution rate curve to a more conventional pattern. The maximum specific growth rate (mu(max)) could not be calculated from the biofilm cultures. However using the sand cultures, mu(max) was determined to 0.64 h(-1) with L-asparagine as the carbon source and 0.49 h(-1) with phenol which compare favorably with the respective mu(max) values calculated from batch cultures.Incorporation of sand into strongly agitated cultures is recommended as an efficient and simple means of controlling biofilm formation in continuous cultures. The method may enable the gathering of basic kinetic data difficult to obtain in the presence of biofilm.
恶臭假单胞菌ATCC 11172在恒化器中以L-天冬酰胺或苯酚作为唯一的限制性碳源和能源进行培养。将存在生物膜的培养物的生长特性与通过悬浮在培养物中的沙子的研磨和剪切作用使生物膜大量减少的培养物的生长特性进行比较。在完整生物膜存在的情况下，稳态生物量与稀释率的曲线与传统恒化器模型预测的理论模式有很大差异。沙子强烈抑制生物膜形成，并在很大程度上使生物量与稀释率曲线的形状恢复到更传统的模式。无法从生物膜培养物中计算出最大比生长速率（μ(max)）。然而，使用沙子培养物，以L-天冬酰胺作为碳源时，μ(max)测定为0.64 h⁻¹，以苯酚作为碳源时为0.49 h⁻¹，这与从分批培养物中计算出的各自的μ(max)值相比具有优势。建议将沙子加入到剧烈搅拌的培养物中，作为控制连续培养中生物膜形成的一种有效且简单的方法。该方法可能有助于获取在生物膜存在时难以获得的基本动力学数据。
