Sharov Victor S, Dremina Elena S, Pennington Justin, Killmer Jacque, Asmus Christopher, Thorson Maria, Hong Sung Jung, Li Xiaobao, Stobaugh John F, Schöneich Christian
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA.
Methods Enzymol. 2008;441:19-32. doi: 10.1016/S0076-6879(08)01202-0.
There is a need for the selective derivatization and enrichment of posttranslational protein modifications from tissue samples. This chapter describes a method for the selective derivatization of 3-nitrotyrosine (after reduction to 3-amino-tyrosine) and 3,4-dihydroxyphenylalanine with benzylamine derivatives to yield 6-amino- and 6-benzylamine-substituted benzoxazoles, which display characteristic fluorescence properties. The methodology can be expanded to other substituted benzylamines, which carry functional groups for affinity enrichment.
需要从组织样本中对翻译后蛋白质修饰进行选择性衍生化和富集。本章描述了一种用苄胺衍生物对3-硝基酪氨酸（还原为3-氨基酪氨酸后）和3,4-二羟基苯丙氨酸进行选择性衍生化的方法，以生成具有特征荧光特性的6-氨基和6-苄胺取代的苯并恶唑。该方法可扩展到其他带有用于亲和富集官能团的取代苄胺。
