Heywood H K, Lee D A
School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London E14NS, UK.
Biochem Biophys Res Commun. 2008 Aug 22;373(2):224-9. doi: 10.1016/j.bbrc.2008.06.011. Epub 2008 Jun 12.
This study tests the hypothesis that articular chondrocytes shift from a characteristically glycolytic to an oxidative energy metabolism during population expansion in monolayer. Bovine articular chondrocytes were cultured in monolayer under standard incubator conditions for up to 14 days. Cellular proliferation, oxygen consumption, lactate production, protein content, ROS generation and mitochondrial morphology were examined. Lactate release increased approximately 5-fold within 1 week, but this was limited to approximately 2-fold increase when normalized to cellular protein content. By contrast, per cell oxidative phosphorylation increased 98-fold in 1 week. The increase in oxidative phosphorylation was evident within 24h, preceding cell proliferation and was associated with augmented reactive oxygen species generation. The autologous chondrocyte implantation procedure requires 14-21 days for population expansion. The alterations in metabolic phenotype we report within 7 days in vitro are thus pertinent to autologous chondrocyte implantation with significant implications for the chondrocyte functionality.
在单层培养的细胞增殖过程中，关节软骨细胞从典型的糖酵解能量代谢转变为氧化能量代谢。将牛关节软骨细胞在标准培养箱条件下进行单层培养，最长培养14天。检测细胞增殖、耗氧量、乳酸生成、蛋白质含量、活性氧生成及线粒体形态。乳酸释放量在1周内增加了约5倍，但以细胞蛋白质含量进行标准化后，仅增加约2倍。相比之下，每细胞的氧化磷酸化在1周内增加了98倍。氧化磷酸化的增加在24小时内就很明显，早于细胞增殖，并与活性氧生成增加有关。自体软骨细胞植入程序需要14 - 21天进行细胞增殖。因此，我们在体外7天内报告的代谢表型变化与自体软骨细胞植入相关，对软骨细胞功能有重要影响。
