Prescott A R, Webb S F, Rawlins D, Shaw P J, Warn R M
Cell Biology Group, School of Biological Sciences, University of East Anglia, Norwich, U.K.
Exp Eye Res. 1991 Jun;52(6):743-53. doi: 10.1016/0014-4835(91)90026-b.
Isolated frog lens epithelia were stained with antibodies against tyrosinated, detyrosinated or acetylated alpha-tubulin and observed by several means including a scanning confocal microscope. The most prominent feature of Rana pipiens lens cells was a primary cilium close to the apical surface of the cells above the centrosome. This structure was associated with microtubules rich in modified alpha-tubulin. The cilium was less pronounced but still discernible in the cells of another species R. ridibunda. In both species, the modified (acetylated or detyrosinated) microtubules formed arrays spatially distinct from the unmodified (tyrosinated) microtubules. The modified microtubules formed a basket of microtubules with a curly distribution around the nucleus while the tyrosinated array consisted predominantly of rather straighter microtubules running from the apical centrosome to the cell periphery, down the lateral sides of the cells and across the basal surface adjacent to the lens capsule and basement membrane. It is concluded that the organization of modified microtubules previously described for several types of cultured cells may represent a remnant of the three-dimensional perinuclear array of such microtubules described here for the cells of an intact epithelium.
将分离的青蛙晶状体上皮细胞用抗酪氨酸化、去酪氨酸化或乙酰化α-微管蛋白的抗体进行染色，并通过多种方法观察，包括扫描共聚焦显微镜。牛蛙晶状体细胞最显著的特征是在中心体上方靠近细胞顶端表面处有一根初级纤毛。这种结构与富含修饰α-微管蛋白的微管相关。在另一种泽蛙的细胞中，纤毛不太明显但仍可辨认。在这两个物种中，修饰（乙酰化或去酪氨酸化）的微管形成的阵列在空间上与未修饰（酪氨酸化）的微管不同。修饰的微管形成一个微管篮，围绕细胞核呈卷曲分布，而酪氨酸化的阵列主要由相当直的微管组成，这些微管从顶端中心体延伸到细胞周边，沿着细胞侧面下行并穿过与晶状体囊膜和基底膜相邻的基底表面。得出的结论是，先前针对几种类型培养细胞所描述的修饰微管的组织方式，可能代表了此处为完整上皮细胞所描述的此类微管三维核周阵列的残余。
