富含去酪氨酸化微管蛋白的高尔基体与微管的时空共定位。
Spatial and temporal colocalization of the Golgi apparatus and microtubules rich in detyrosinated tubulin.
作者信息
Skoufias D A, Burgess T L, Wilson L
机构信息
Department of Biological Sciences, University of California, Santa Barbara 93106.
出版信息
J Cell Biol. 1990 Nov;111(5 Pt 1):1929-37. doi: 10.1083/jcb.111.5.1929.
Abstract
The integrity and intracellular distribution of the Golgi apparatus appear to depend upon microtubules. We have found that the microtubules rich in detyrosinated tubulin are located preferentially in the vicinity of the Golgi. Cells were double-stained with antibodies specific for either tyrosinated or detyrosinated tubulin and an antibody to prolactin or wheat germ agglutinin (Golgi markers). Microtubules rich in detyrosinated tubulin showed a close codistribution with the Golgi in three different cultured cell lines GH3, BS-C-1, and AtT20. Disruption of microtubules with nocodazole in GH3 cells resulted in fragmentation and dispersal of the Golgi apparatus as reported previously. During recovery of the microtubules and the Golgi complex after removal of the nocodazole, there was a spatial and temporal colocalization of the Golgi apparatus and microtubules rich in detyrosinated tubulin. Our results suggest that a functional relationship may exist between the structure and organization of the Golgi complex and the detyrosination of alpha-tubulin in microtubules.
摘要
高尔基体的完整性及其在细胞内的分布似乎依赖于微管。我们发现富含去酪氨酸化微管蛋白的微管优先位于高尔基体附近。用针对酪氨酸化或去酪氨酸化微管蛋白的特异性抗体以及催乳素抗体或麦胚凝集素（高尔基体标记物）对细胞进行双重染色。富含去酪氨酸化微管蛋白的微管在三种不同的培养细胞系GH3、BS-C-1和AtT20中与高尔基体呈现紧密的共分布。如先前报道的那样，用诺考达唑破坏GH3细胞中的微管会导致高尔基体的碎片化和分散。在去除诺考达唑后微管和高尔基体复合体恢复的过程中，高尔基体与富含去酪氨酸化微管蛋白的微管在空间和时间上存在共定位。我们的结果表明，高尔基体复合体的结构和组织与微管中α-微管蛋白的去酪氨酸化之间可能存在功能关系。
相似文献
1
Spatial and temporal colocalization of the Golgi apparatus and microtubules rich in detyrosinated tubulin.富含去酪氨酸化微管蛋白的高尔基体与微管的时空共定位。
J Cell Biol. 1990 Nov;111(5 Pt 1):1929-37. doi: 10.1083/jcb.111.5.1929.
2
Relationship between the Golgi complex and microtubules enriched in detyrosinated or acetylated alpha-tubulin: studies on cells recovering from nocodazole and cells in the terminal phase of cytokinesis.富含去酪氨酸化或乙酰化α-微管蛋白的高尔基体复合体与微管之间的关系：对从诺考达唑恢复的细胞和处于胞质分裂末期的细胞的研究
Cell Tissue Res. 1993 Sep;273(3):457-66. doi: 10.1007/BF00333700.
3
Disruption of the Golgi apparatus with brefeldin A does not destabilize the associated detyrosinated microtubule network.用布雷菲德菌素A破坏高尔基体不会使相关的去酪氨酸化微管网络不稳定。
Cell Motil Cytoskeleton. 1991;20(4):289-300. doi: 10.1002/cm.970200405.
4
Confocal analysis of primary cilia structure and colocalization with the Golgi apparatus in chondrocytes and aortic smooth muscle cells.软骨细胞和主动脉平滑肌细胞中原发性纤毛结构及与高尔基体共定位的共聚焦分析。
Cell Biol Int. 1997 Aug;21(8):483-94. doi: 10.1006/cbir.1997.0177.
5
Redistribution of microtubules and Golgi apparatus in herpes simplex virus-infected cells and their role in viral exocytosis.单纯疱疹病毒感染细胞中微管和高尔基体的重新分布及其在病毒胞吐作用中的作用。
J Virol. 1995 Dec;69(12):7472-82. doi: 10.1128/JVI.69.12.7472-7482.1995.
6
Individual microtubules in the axon consist of domains that differ in both composition and stability.轴突中的单个微管由在组成和稳定性方面都不同的结构域组成。
J Cell Biol. 1990 Aug;111(2):495-509. doi: 10.1083/jcb.111.2.495.
7
Role of microtubules in the organization and localization of the Golgi apparatus.微管在高尔基体的组织和定位中的作用。
J Cell Biol. 1984 Jul;99(1 Pt 2):113s-118s. doi: 10.1083/jcb.99.1.113s.
8
Modifications of the Golgi apparatus in Saccharomyces cerevisiae lacking microtubules.酿酒酵母中缺乏微管时高尔基体的修饰。
Anat Rec. 1996 Oct;246(2):162-8. doi: 10.1002/(SICI)1097-0185(199610)246:2<162::AID-AR2>3.0.CO;2-3.
9
Synchronized shift in localization of the Golgi complex and the microtubule organizing center in the terminal phase of cytokinesis.胞质分裂末期高尔基体复合体和微管组织中心定位的同步转移。
J Submicrosc Cytol Pathol. 1992 Jul;24(3):359-70.
10
Three-dimensional organization of stable microtubules and the Golgi apparatus in the somata of developing chick sensory neurons.发育中鸡感觉神经元胞体中稳定微管和高尔基体的三维组织
J Neurocytol. 1995 Mar;24(3):207-23. doi: 10.1007/BF01181535.
引用本文的文献
1
TREX1 Deficiency Induces ER Stress-Mediated Neuronal Cell Death by Disrupting Ca Homeostasis.TREX1 缺乏通过破坏钙稳态诱导内质网应激介导的神经元细胞死亡。
Mol Neurobiol. 2022 Mar;59(3):1398-1418. doi: 10.1007/s12035-021-02631-3. Epub 2022 Jan 7.
2
Microtubules: Evolving roles and critical cellular interactions.微管：不断演变的角色和关键细胞相互作用。
Exp Biol Med (Maywood). 2019 Nov;244(15):1240-1254. doi: 10.1177/1535370219867296. Epub 2019 Aug 6.
3
Intraflagellar transport 20 promotes collective cancer cell invasion by regulating polarized organization of Golgi-associated microtubules.鞭毛内运输 20 通过调节与高尔基体相关的微管的极化组织促进细胞的集体侵袭。
Cancer Sci. 2019 Apr;110(4):1306-1316. doi: 10.1111/cas.13970. Epub 2019 Feb 28.
4
The Protein Encoded by the CCDC170 Breast Cancer Gene Functions to Organize the Golgi-Microtubule Network.CCDC170 乳腺癌基因编码的蛋白可用于构建高尔基体-微管网络。
EBioMedicine. 2017 Aug;22:28-43. doi: 10.1016/j.ebiom.2017.06.024. Epub 2017 Jun 27.
5
Stathmin 1/2-triggered microtubule loss mediates Golgi fragmentation in mutant SOD1 motor neurons.Stathmin 1/2引发的微管丢失介导了突变型SOD1运动神经元中的高尔基体碎片化。
Mol Neurodegener. 2016 Jun 9;11(1):43. doi: 10.1186/s13024-016-0111-6.
6
Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration.桩蛋白抑制组蛋白去乙酰化酶6以调节微管乙酰化、高尔基体结构和极化迁移。
J Cell Biol. 2014 Aug 4;206(3):395-413. doi: 10.1083/jcb.201403039. Epub 2014 Jul 28.
7
Microtubule stability, Golgi organization, and transport flux require dystonin-a2-MAP1B interaction.微管稳定性、高尔基体组织和运输通量需要 dystonin-a2-MAP1B 相互作用。
J Cell Biol. 2012 Mar 19;196(6):727-42. doi: 10.1083/jcb.201107096. Epub 2012 Mar 12.
8
Vinblastine and nocodazole inhibit basal and thyrotropin-releasing hormone-stimulated prolactin secretion in GH(3) cells.
Endocrine. 1995 Aug;3(8):591-6. doi: 10.1007/BF02953024.
9
DAAM1 is a formin required for centrosome re-orientation during cell migration.DAAM1 是一种在细胞迁移过程中重新定向中心体所必需的formin。
PLoS One. 2010 Sep 29;5(9):e13064. doi: 10.1371/journal.pone.0013064.
10
The differential distribution of acetylated and detyrosinated alpha-tubulin in the microtubular cytoskeleton and primary cilia of hyaline cartilage chondrocytes.乙酰化和去酪氨酸化α-微管蛋白在透明软骨软骨细胞微管细胞骨架和初级纤毛中的差异分布。
J Anat. 2001 Oct;199(Pt 4):393-405. doi: 10.1046/j.1469-7580.2001.19940393.x.
本文引用的文献
1
BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY.源自非洲绿猴的连续肾细胞系的生物学特性
J Immunol. 1963 Sep;91:416-24.
2
ACTH secreting transplantable pituitary tumors.分泌促肾上腺皮质激素的可移植垂体瘤
Proc Soc Exp Biol Med. 1953 Oct;84(1):253-4. doi: 10.3181/00379727-84-20607.
3
A monoclonal antibody against a 135-K Golgi membrane protein.一种针对135K高尔基体膜蛋白的单克隆抗体。
EMBO J. 1982;1(12):1621-8. doi: 10.1002/j.1460-2075.1982.tb01364.x.
4
Modulation of some parameters of assembly of microtubules in vitro by tyrosinolation of tubulin.通过微管蛋白的酪氨酸化对体外微管组装的一些参数进行调节。
Eur J Biochem. 1982 Nov;128(1):215-22. doi: 10.1111/j.1432-1033.1982.tb06954.x.
5
Polarization of the Golgi apparatus and the microtubule-organizing center in cultured fibroblasts at the edge of an experimental wound.实验伤口边缘培养的成纤维细胞中高尔基体和微管组织中心的极化。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2603-7. doi: 10.1073/pnas.79.8.2603.
6
Golgi detection in mitotic and interphase cells by antibodies to secreted galactosyltransferase.通过分泌型半乳糖基转移酶抗体对有丝分裂细胞和间期细胞中的高尔基体进行检测。
Exp Cell Res. 1982 Nov;142(1):85-94. doi: 10.1016/0014-4827(82)90412-8.
7
A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate filaments, and arrangement of Golgi elements.一种与α-微管蛋白的酪氨酸化形式特异性反应的大鼠单克隆抗体。II. 对细胞运动、微管组织、中间丝以及高尔基体元件排列的影响。
J Cell Biol. 1983 Nov;97(5 Pt 1):1476-90. doi: 10.1083/jcb.97.5.1476.
8
Distinct populations of microtubules: tyrosinated and nontyrosinated alpha tubulin are distributed differently in vivo.不同的微管群体：酪氨酸化和非酪氨酸化的α微管蛋白在体内分布不同。
Cell. 1984 Oct;38(3):779-89. doi: 10.1016/0092-8674(84)90273-3.
9
Associations of elements of the Golgi apparatus with microtubules.高尔基体的组成成分与微管的关联。
J Cell Biol. 1984 Sep;99(3):1092-100. doi: 10.1083/jcb.99.3.1092.
10
Lectin-binding sites as markers of Golgi subcompartments: proximal-to-distal maturation of oligosaccharides.凝集素结合位点作为高尔基体亚区室的标记：寡糖从近端到远端的成熟过程
J Cell Biol. 1983 Oct;97(4):1243-8. doi: 10.1083/jcb.97.4.1243.
Zotero 插件