Skoufias D A, Burgess T L, Wilson L
Department of Biological Sciences, University of California, Santa Barbara 93106.
J Cell Biol. 1990 Nov;111(5 Pt 1):1929-37. doi: 10.1083/jcb.111.5.1929.
The integrity and intracellular distribution of the Golgi apparatus appear to depend upon microtubules. We have found that the microtubules rich in detyrosinated tubulin are located preferentially in the vicinity of the Golgi. Cells were double-stained with antibodies specific for either tyrosinated or detyrosinated tubulin and an antibody to prolactin or wheat germ agglutinin (Golgi markers). Microtubules rich in detyrosinated tubulin showed a close codistribution with the Golgi in three different cultured cell lines GH3, BS-C-1, and AtT20. Disruption of microtubules with nocodazole in GH3 cells resulted in fragmentation and dispersal of the Golgi apparatus as reported previously. During recovery of the microtubules and the Golgi complex after removal of the nocodazole, there was a spatial and temporal colocalization of the Golgi apparatus and microtubules rich in detyrosinated tubulin. Our results suggest that a functional relationship may exist between the structure and organization of the Golgi complex and the detyrosination of alpha-tubulin in microtubules.
高尔基体的完整性及其在细胞内的分布似乎依赖于微管。我们发现富含去酪氨酸化微管蛋白的微管优先位于高尔基体附近。用针对酪氨酸化或去酪氨酸化微管蛋白的特异性抗体以及催乳素抗体或麦胚凝集素（高尔基体标记物）对细胞进行双重染色。富含去酪氨酸化微管蛋白的微管在三种不同的培养细胞系GH3、BS-C-1和AtT20中与高尔基体呈现紧密的共分布。如先前报道的那样，用诺考达唑破坏GH3细胞中的微管会导致高尔基体的碎片化和分散。在去除诺考达唑后微管和高尔基体复合体恢复的过程中，高尔基体与富含去酪氨酸化微管蛋白的微管在空间和时间上存在共定位。我们的结果表明，高尔基体复合体的结构和组织与微管中α-微管蛋白的去酪氨酸化之间可能存在功能关系。
