Ha Y, Ahn K K, Kim B, Cho K-D, Lee B H, Oh Y-S, Kim S-H, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
Res Vet Sci. 2009 Feb;86(1):108-10. doi: 10.1016/j.rvsc.2008.04.004. Epub 2008 Jun 13.
Detection of porcine circovirus type 2 (PCV2) was to evaluate the milk from experimentally infected sows using polymerase chain reaction (PCR) and virus isolation. Six pregnant sows were inoculated intranasally with PCV2 at 93 days of gestation, and milk samples were collected from all sows at 1, 3, 6, 9, 12, 15, 18, 21, 24, and 27 days of lactation. PCV2 was detected in milk as early as day 1 of lactation in all six sows. Thereafter, all infected sows remained positive by PCR for PCV2 in milk until 27 days of lactation. In addition, PCV2 itself was isolated from milk collected from a virus-infected sows. These results suggest that PCV2 may be shed in milk following infection of pregnant sows by the virus.
为了评估实验感染母猪的乳汁中猪圆环病毒2型(PCV2)的情况,采用聚合酶链反应(PCR)和病毒分离技术进行检测。6头怀孕母猪在妊娠93天时经鼻接种PCV2,在泌乳的第1、3、6、9、12、15、18、21、24和27天从所有母猪采集乳汁样本。所有6头母猪在泌乳第1天的乳汁中就检测到了PCV2。此后,所有感染母猪的乳汁经PCR检测PCV2一直呈阳性,直至泌乳第27天。此外,从一头病毒感染母猪采集的乳汁中分离出了PCV2本身。这些结果表明,怀孕母猪感染该病毒后,PCV2可能会随乳汁排出。