Park J-S, Ha Y, Kwon B, Cho K D, Lee B H, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
J Comp Pathol. 2009 Feb-Apr;140(2-3):208-11. doi: 10.1016/j.jcpa.2008.11.004. Epub 2009 Jan 4.
The aim of this study was to determine whether porcine circovirus 2 (PCV2) may infect the mammary gland of sows and be shed in the milk. Six pregnant sows were inoculated intranasally with PCV2 three weeks before their expected farrowing date and two further sows acted as uninfected controls. The animals remained clinically healthy and farrowed normally. Milk samples were collected from all sows on the first, second and third days of lactation. PCV2 DNA was detected in the milk of infected sows from day 1 of lactation but not in the milk of uninfected controls. PCV2 antigen and DNA were detected in the mammary gland and other tissues by immunohistochemistry and in-situ hybridization, respectively. Simultaneous detection of viral protein and DNA provided molecular evidence of PCV2 infection and replication within these tissues.
本研究的目的是确定猪圆环病毒2型（PCV2）是否可能感染母猪的乳腺并随乳汁排出。在预计分娩日期前三周，对六头怀孕母猪进行鼻内接种PCV2，另外两头母猪作为未感染对照。这些动物临床保持健康并正常产仔。在泌乳的第一天、第二天和第三天从所有母猪采集乳汁样本。从泌乳第1天起在感染母猪的乳汁中检测到PCV2 DNA，但在未感染对照的乳汁中未检测到。分别通过免疫组织化学和原位杂交在乳腺和其他组织中检测到PCV2抗原和DNA。病毒蛋白和DNA的同时检测提供了PCV2在这些组织内感染和复制的分子证据。
