Perpiña M, Cortijo J, Fornás E, Palau M, Ortiz J L, Morcillo E
Service of Pneumology, Hospital La Fé, Valencia, Spain.
Eur Respir J. 1991 Apr;4(4):450-7.
Responses to KCl and histamine and 45Ca movements were studied in trachea from normal and actively sensitized guinea-pigs. Sensitized tracheas were hyperresponsive and hypersensitive to KCl and histamine. 45Ca uptake experiments show that sensitized tracheal muscle behaves as normal except that the uptake of 45Ca in low concentration (0.03 mM) Ca2+ solution was higher and the number of binding sites for the high affinity component of 45Ca uptake (as estimated by Scatchard-coordinate plot) was augmented. Additionally, in sensitized tracheal muscle, incubation in low (0.03 mM) Ca2+ solution followed by La3+ wash-out resulted in a greater amount of residual 45Ca than in normal tissues. KCl, but not histamine, increased the La3+ resistant 45Ca content. This increase was greater in sensitized than in normal trachea. This demonstrates the existence of hyperreactivity and altered 45Ca movements in sensitized trachealis muscle.
对正常和主动致敏豚鼠的气管对氯化钾、组胺的反应以及45钙的移动进行了研究。致敏气管对氯化钾和组胺反应过度且敏感。45钙摄取实验表明，致敏气管肌肉的表现与正常情况相同，只是在低浓度（0.03 mM）钙离子溶液中45钙的摄取量更高，并且45钙摄取高亲和力成分的结合位点数量（通过Scatchard坐标图估算）增加。此外，在致敏气管肌肉中，在低（0.03 mM）钙离子溶液中孵育后用镧离子洗脱，残留的45钙量比正常组织更多。氯化钾而非组胺增加了镧离子抗性45钙的含量。致敏气管中这种增加比正常气管更大。这证明了致敏气管肌存在反应过度和45钙移动改变的情况。
