Elsner J
Department of Biophysics and Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland.
Plant Biol (Stuttg). 2008 Jul;10(4):422-32. doi: 10.1111/j.1438-8677.2008.00052.x.
Orientation of cortical microtubules (cMTs) is suggested to be affected by mechanical stress existing in cell walls. However, in mutants exhibiting helical (chiral) growth, there is a correlation between orientation of cMTs in outer tissues and helical growth direction. The aim of this research was to examine the effect of a chiral mechanical stimulation on cMTs. For this purpose, the orientation of cMTs was investigated in hypocotyls subjected to either a right- or a left-handed twist, resulting from a steady torque. cMTs were visualised in fixed material using the immunofluorescence method. The cMTs in untouched control hypocotyls were mostly transverse with respect to the cell long axis. In immobilised, but not twisted control hypocotyls, the transverse orientation was also most frequent, while applied twisting resulted in a change in cMT orientation from transverse to oblique. The data provide additional evidence that changes in tissue stress can be reorganized by cortical microtubules.
有研究表明，细胞壁中存在的机械应力会影响皮层微管（cMTs）的方向。然而，在表现出螺旋（手性）生长的突变体中，外部组织中cMTs的方向与螺旋生长方向之间存在相关性。本研究的目的是研究手性机械刺激对cMTs的影响。为此，研究了在稳定扭矩作用下，经右旋或左旋扭转处理的下胚轴中cMTs的方向。使用免疫荧光法在固定材料中观察cMTs。未处理的对照下胚轴中的cMTs大多与细胞长轴呈横向排列。在固定但未扭转的对照下胚轴中，横向排列也最为常见，而施加扭转会导致cMTs方向从横向变为倾斜。这些数据提供了额外的证据，表明组织应力的变化可以通过皮层微管进行重新组织。
