Stögbauer Fabian, Neumeier Markus, Weigert Johanna, Wanninger Josef, Grandl Margot, Lehle Karla, Schmitz Gerd, Aslanidis Charalampos, Schäffler Andreas, Schölmerich Jürgen, Buechler Christa
Department of Internal Medicine I, University of Regensburg, Regensburg, Germany.
J Immunol Methods. 2008 Aug 20;337(1):78-80. doi: 10.1016/j.jim.2008.05.008. Epub 2008 Jun 4.
Several techniques are available to purify circulating blood monocytes for research. CD14-containing MicroBeads are suitable and reliable tools to reproducibly isolate human monocytes with a high purity but are quite expensive. This report describes that a comparable number of highly pure monocytes can be isolated from samples using up to tenfold lower amounts of CD14-MicroBeads. MicroBeads are widely used to isolate different cell populations and with this report more researchers may be encouraged to use this highly efficient, low-cost and thus affordable method to pursue their scientific goals.
