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在尼日利亚北部地区,一种新型经济适用的流式细胞术方法与手动磁珠技术用于CD4 T淋巴细胞计数的比较。

Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting.

作者信息

Imade Godwin E, Badung Bitrus, Pam Sunday, Agbaji Oche, Egah Daniel, Sagay Atiene S, Sankalé Jean-Louis, Kapiga Saidi, Idoko John, Kanki Phyllis

机构信息

AIDS Prevention Initiative Nigeria, Jos University Teaching Hospital, Jos, Nigeria.

出版信息

Clin Diagn Lab Immunol. 2005 Jan;12(1):224-7. doi: 10.1128/CDLI.12.1.224-227.2005.

Abstract

We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P=0.057, r=0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.

摘要

我们比较了两种用于CD4 T淋巴细胞计数的技术:流式细胞术(Cyflow)和磁珠法(Dynabead)。对40份成人血样计数后得到了具有良好相关性的相似结果(P = 0.057,r = 0.93)。Cyflow技术比Dynabead方法更精确且更具成本效益(每次检测分别为3美元至5美元和12美元至22美元),因为每天可检测多达200个样本。

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