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用于分离嗜麦芽窄食单胞菌的改良选择性鉴别培养基的评估

Evaluation of a modified selective differential medium for the isolation of Stenotrophomonas maltophilia.

作者信息

Foster Niki F, Chang Barbara J, Riley Thomas V

机构信息

Discipline of Microbiology and Immunology, School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Australia.

出版信息

J Microbiol Methods. 2008 Sep;75(1):153-5. doi: 10.1016/j.mimet.2008.05.003. Epub 2008 May 13.

Abstract

VIA medium for Stenotrophomonas maltophilia was modified by substituting meropenem (16 mg/L) for imipenem. S. maltophilia grew from 40% of drains sampled within a hospital and surrounding locations in Perth, Western Australia. The specificity of the new medium for S. maltophilia was 62%, and all contaminating bacteria were easily distinguishable by phenotypic tests and PCR.

摘要

嗜麦芽窄食单胞菌的VIA培养基通过用美罗培南(16毫克/升)替代亚胺培南进行了改良。在澳大利亚西部珀斯一家医院及其周边地区采集的引流液样本中,40%检测出嗜麦芽窄食单胞菌生长。新培养基对嗜麦芽窄食单胞菌的特异性为62%,所有污染细菌均可通过表型试验和聚合酶链反应轻松区分。

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