一种鉴定嗜麦芽寡养单胞菌的特定聚合酶链反应方法。
A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia.
机构信息
Pontifícia Universidade Católica do Rio Grande do Su, Faculdade de Biociências, Departamento de Biologia Celular e Molecular, Laboratório de Imunologia e Microbiologia, Porto Alegre, RS, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2013 May;108(3):390-1. doi: 10.1590/S0074-02762013000300020.
Abstract
Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
摘要
嗜麦芽寡养单胞菌是一种多药耐药的医院获得性病原体，目前的方法难以明确鉴定。因此，由于患者体内存在这种微生物可能直接决定抗菌治疗方案，因此针对 23S rRNA 开发了常规聚合酶链反应（PCR）和实时 PCR 检测方法，用于嗜麦芽寡养单胞菌的特异性鉴定。该 PCR 方案在针对其他嗜麦芽寡养单胞菌种、非发酵革兰氏阴性杆菌以及先前使用 Vitek 系统鉴定的 100 株嗜麦芽寡养单胞菌临床分离株进行检测时显示出很高的特异性。
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