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芒果(芒果属印度种)树皮、果仁、树叶和果皮中多酚类化合物的表征与定量分析。

Characterization and quantitation of polyphenolic compounds in bark, kernel, leaves, and peel of mango (Mangifera indica L.).

作者信息

Barreto Jacqueline C, Trevisan Maria T S, Hull William E, Erben Gerhard, de Brito Edy S, Pfundstein Beate, Würtele Gerd, Spiegelhalder Bertold, Owen Robert W

机构信息

German Cancer Research Center, Heidelberg, Germany.

出版信息

J Agric Food Chem. 2008 Jul 23;56(14):5599-610. doi: 10.1021/jf800738r. Epub 2008 Jun 18.

DOI:10.1021/jf800738r
PMID:18558692
Abstract

The contents of secondary plant substances in solvent extracts of various byproducts (barks, kernels, peels, and old and young leaves) in a range of Brazilian mango cultivars were identified and quantitated. The results show that the profiles of secondary plant substances such as xanthone C-glycosides, gallotannins, and benzophenones in different byproducts vary greatly but are fairly consistent across cultivars. The free radical scavenging activity of the solvent extracts was evaluated using a high-performance liquid chromatography-based hypoxanthine/xanthine oxidase assay and revealed dose-dependent antioxidant capacity in all extracts. Four (mangiferin, penta- O-galloyl-glucoside gallic acid, and methyl gallate) of the major phenolic compounds detected were also evaluated in additional in vitro bioassay systems such as oxygen radical absorbance capacity, 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing ability of plasma. Mangiferin in particular, detected at high concentrations in young leaves (Coite = 172 g/kg), in bark (Momika = 107 g/kg), and in old leaves (Itamaraka = 94 g/kg), shows an exceptionally strong antioxidant capacity.

摘要

对一系列巴西芒果品种的各种副产品(树皮、果核、果皮以及老叶和嫩叶)的溶剂提取物中的次生植物物质含量进行了鉴定和定量分析。结果表明,不同副产品中次生植物物质(如氧杂蒽酮C-糖苷、没食子单宁和二苯甲酮)的概况差异很大,但在不同品种间相当一致。使用基于高效液相色谱的次黄嘌呤/黄嘌呤氧化酶测定法评估了溶剂提取物的自由基清除活性,结果显示所有提取物均具有剂量依赖性抗氧化能力。还在其他体外生物测定系统(如氧自由基吸收能力、2,2-二苯基-1-苦基肼基和血浆铁还原能力)中对检测到的四种主要酚类化合物(芒果苷、五-O-没食子酰葡萄糖、没食子酸和没食子酸甲酯)进行了评估。特别是在嫩叶(Coite品种 = 172 g/kg)、树皮(Momika品种 = 107 g/kg)和老叶(Itamaraka品种 = 94 g/kg)中高浓度检测到的芒果苷,显示出极强的抗氧化能力。

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