Mariod Abdalbasit Adam, Abdelwahab Siddig Ibrahim, Elkheir Sara, Ahmed Yousif Mohamed, Fauzi Putri Narrima Mohd, Chuen Cheah Shiau
Food Science and Technology Department, College of Agricultural Studies, Sudan University of Science and Technology P.O. Box 71 Khartoum North, Sudan.
Acta Sci Pol Technol Aliment. 2012 Jul-Sep;11(3):249-58.
We extracted phenolic compounds from Annona squamosa (leaves, bark, roots and seedcake), and Catunaregam nilotica (leaves, bark and seedcake) using methanol and their antioxidant activity was evaluated employing various established in vitro systems.
Annona squamosa (leaves, bark, roots and seedcake), and Catunaregam nilotica (leaves, bark and seedcake) were used in the study. Antioxidant activity was estimated using oxygen radical absorbance capacity, MTT assay and DPPH assays, and polyphenols profile was determined by HPLC method.
The total phenolic content was determined by Folin-Ciocalteu method and the highest amounts were 171.5, 170.4, 169.5, and 167.9 g/kg plant extract as GAE for A. squamosa roots, C. nilotica bark, C. nilotica leaves, and A. squamosa bark, respectively. The leaves extracts of the two trees showed high flavoniod content. The results showed that C. nilotica and A. squamosa extracts displayed antioxidant activities, with IC(50) values ranging from 7.81 to 62.5 and from 7.81 to 125.0 μg/ml, respectively using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The different parts extracts from two trees showed good antioxidant activity evaluated by oxygen radical absorbance capacity and MTT assay systems.
These results suggested that Annona squamosa and Catunaregam nilotica phenolic compounds could be utilized as a natural antioxidant.
我们使用甲醇从番荔枝（叶、树皮、根和籽饼）以及水团花（叶、树皮和籽饼）中提取了酚类化合物，并采用各种已建立的体外系统评估了它们的抗氧化活性。
本研究使用了番荔枝（叶、树皮、根和籽饼）以及水团花（叶、树皮和籽饼）。通过氧自由基吸收能力、MTT 法和 DPPH 法评估抗氧化活性，并采用高效液相色谱法测定多酚谱。
采用福林 - 酚法测定总酚含量，以没食子酸当量计，番荔枝根、水团花树皮、水团花叶和番荔枝树皮的植物提取物中总酚含量最高，分别为 171.5、170.4、169.5 和 167.9 g/kg。两种树的叶提取物显示出较高的黄酮含量。结果表明，使用 1,1 - 二苯基 - 2 - 苦基肼（DPPH）法，水团花和番荔枝提取物表现出抗氧化活性，IC(50)值分别为 7.81 至 62.5 μg/ml 和 7.81 至 125.0 μg/ml。通过氧自由基吸收能力和 MTT 法评估，两种树不同部位的提取物均显示出良好的抗氧化活性。
这些结果表明，番荔枝和水团花的酚类化合物可作为天然抗氧化剂使用。
