Steyer Andrej, Poljšak-Prijatelj Mateja, Barlič-Maganja Darja, Marin Jožica
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, SI-1104 Ljubljana, Slovenia.
College of Health Care, University of Primorska, Polje 42, SI-6310 Izola, Slovenia.
J Gen Virol. 2008 Jul;89(Pt 7):1690-1698. doi: 10.1099/vir.0.2008/001206-0.
A surveillance of human, porcine and bovine rotaviruses was carried out in Slovenia in 2004 and 2005. Stool samples were collected from a total of 406 pigs (373 from asymptomatic animals), 132 cattle (126 from asymptomatic animals) and 241 humans (all with diarrhoea), tested for group A rotaviruses using RT-PCR and analysed by sequencing. The aims of the study were to determine the incidence of asymptomatic rotavirus infection in animals, to look for evidence of zoonotic transmission and to detect reassortment among rotaviruses. The rates of asymptomatic shedding of rotaviruses in pigs and cattle were 18.0 % (67/373) and 4.0 % (5/126), respectively. Evidence for zoonotic transmission was detected in one human rotavirus strain, SI-MB6, with the G3P[6] genotype combination, as the nucleotide and predicted amino acid sequences of the VP6, VP7, VP8* and NSP4 genes of strain SI-MB6 and of porcine strains showed high nucleotide and amino acid sequence identity. Two porcine rotavirus strains carried VP7 of probable human origin, suggesting an interspecies reassortment event in the past.
2004年和2005年在斯洛文尼亚开展了一项针对人、猪和牛轮状病毒的监测。总共从406头猪（373头来自无症状动物）、132头牛（126头来自无症状动物）和241人（均患有腹泻）采集粪便样本，使用逆转录聚合酶链反应（RT-PCR）检测A组轮状病毒，并通过测序进行分析。该研究的目的是确定动物中无症状轮状病毒感染的发生率，寻找人畜共患传播的证据，并检测轮状病毒之间的重配。猪和牛中轮状病毒无症状排毒率分别为18.0%（67/373）和4.0%（5/126）。在一株人轮状病毒SI-MB6（G3P[6]基因型组合）中检测到了人畜共患传播的证据，因为SI-MB6株与猪株的VP6、VP7、VP8*和NSP4基因的核苷酸和预测氨基酸序列显示出高度的核苷酸和氨基酸序列同一性。两株猪轮状病毒携带可能源自人类的VP7，表明过去发生了种间重配事件。
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