Mukherjee Anupam, Dutta Dipanjan, Ghosh Souvik, Bagchi Parikshit, Chattopadhyay Shiladitya, Nagashima Shigeo, Kobayashi Nobumichi, Dutta Phalguni, Krishnan Triveni, Naik Trailokya Nath, Chawla-Sarkar Mamta
Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road Scheme XM, Beliaghata, Kolkata 700010, West Bengal, India.
Arch Virol. 2009;154(5):733-46. doi: 10.1007/s00705-009-0363-3. Epub 2009 Mar 31.
Deduced amino acid sequence and phylogenetic analyses of a group A rotavirus G9P[6] strain (designated as mcs/13-07), detected from a 3-year-old child in Eastern India, revealed a VP8* closely related to porcine P[6] strains (P[6] sublineage 1D), and the VP7 clustered with G9 lineage-III strains. To our knowledge, this is the first report of human P[6] strain clustering in sublineage Id. Thus, to further characterize the evolutionary diversity of strain mcs/13-07, all gene segments were analyzed. VP6 and NSP4 exhibited genetic relatedness to Wa-like human subgroup II strains, while VP1-3, NSP1-3 and NSP5 were closely related to porcine strains. Based on the new classification system of rotaviruses, mcs/13-07 revealed a G9-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1 genotype with close similarity to human Wa-like and porcine Gottfried strains. Therefore, considering the porcine-like or porcine origin of multiple gene segments, it might be tempting to assume that strain mcs/13-07 represents a rare instance of whole-virus transmission from pig to human, after which the virus evolved with time. Alternatively, it is possible that strain mcs/13-07 resulted from multiple reassortment events involving human subgroup II and porcine P[6] strains. Nevertheless, detection of strain mcs/13-07 provides further evidence for complex interspecies transmission events, which are frequent in developing countries.
从印度东部一名3岁儿童体内检测出的一株A组轮状病毒G9P[6]毒株（命名为mcs/13 - 07），其推导的氨基酸序列和系统发育分析显示，VP8*与猪P[6]毒株（P[6]亚系1D）密切相关，VP7则与G9谱系III毒株聚类。据我们所知，这是人类P[6]毒株在亚系Id中聚类的首次报道。因此，为进一步表征毒株mcs/13 - 07的进化多样性，对所有基因片段进行了分析。VP6和NSP4与Wa样人类II亚组毒株表现出遗传相关性，而VP1 - 3、NSP1 - 3和NSP5与猪毒株密切相关。基于轮状病毒的新分类系统，mcs/13 - 07显示出G9 - P[6] - I1 - R1 - C1 - M1 - A8 - N1 - T1 - E1 - H1基因型，与人类Wa样和猪Gottfried毒株高度相似。因此，考虑到多个基因片段的猪样或猪源性质，可能会倾向于认为毒株mcs/13 - 07代表了从猪到人的全病毒传播的罕见实例，此后病毒随时间进化。或者，毒株mcs/13 - 07可能是由涉及人类II亚组和猪P[6]毒株的多次重配事件导致的。尽管如此，毒株mcs/13 - 07的检测为发展中国家频繁发生的复杂种间传播事件提供了进一步证据。
