Koga Hideyuki, Muneta Takeshi, Nagase Tsuyoshi, Nimura Akimoto, Ju Young-Jin, Mochizuki Tomoyuki, Sekiya Ichiro
Section of Orthopedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Cell Tissue Res. 2008 Aug;333(2):207-15. doi: 10.1007/s00441-008-0633-5. Epub 2008 Jun 17.
We previously compared mesenchymal stem cells (MSCs) from a variety of mesenchymal tissues and demonstrated that synovium-MSCs had the best expansion and chondrogenic ability in vitro in humans and rats. In this study, we compared the in vivo chondrogenic potential of rabbit MSCs. We also examined other parameters to clarify suitable conditions for in vitro and in vivo cartilage formation. MSCs were isolated from bone marrow, synovium, adipose tissue, and muscle of adult rabbits. Proliferation potential and in vitro chondrogenic potential were compared. Toxicity of the tracer DiI for in vitro chondrogenesis was also examined. MSCs from each tissue were embedded in collagen gel and transplanted into full thickness cartilage defects of rabbits. Cartilage matrix production was compared histologically. The effects of cell density and periosteal patch on the in vivo chondrogenic potential of synovium-MSCs were also examined. Synovium- and muscle-MSCs had a higher proliferation potential than other cells. Pellets from synovium- and bone-marrow-MSCs showed abundant cartilage matrix. DiI had no significant influence on in vitro cartilage formation. After transplantation into cartilage defects, synovium- and bone-marrow-MSCs produced much more cartilage matrix than other cells. When synovium-MSCs were transplanted at a higher cell density and with a periosteal patch, more abundant cartilage matrix was observed. Thus, synovium- and bone-marrow-MSCs had greater in vivo chondrogenic potential than adipose- and muscle-MSCs, but synovium-MSCs had the advantage of a greater proliferation potential. Higher cell density and a periosteum patch were needed to obtain a high production of cartilage matrix by synovium-MSCs.
我们之前比较了来自多种间充质组织的间充质干细胞（MSC），并证明滑膜来源的MSC在体外对人和大鼠具有最佳的增殖及软骨形成能力。在本研究中，我们比较了兔MSC的体内软骨形成潜力。我们还检测了其他参数，以明确体外和体内软骨形成的适宜条件。从成年兔的骨髓、滑膜、脂肪组织和肌肉中分离MSC。比较了其增殖潜力和体外软骨形成潜力。还检测了示踪剂DiI对体外软骨形成的毒性。将各组织来源的MSC包埋于胶原凝胶中，移植到兔的全层软骨缺损处。通过组织学方法比较软骨基质的产生情况。还检测了细胞密度和骨膜补片对滑膜来源MSC体内软骨形成潜力的影响。滑膜和肌肉来源的MSC比其他细胞具有更高的增殖潜力。滑膜和骨髓来源的MSC形成的细胞球显示出丰富的软骨基质。DiI对体外软骨形成无显著影响。移植到软骨缺损处后，滑膜和骨髓来源的MSC比其他细胞产生更多的软骨基质。当以更高的细胞密度并使用骨膜补片移植滑膜来源的MSC时，可观察到更丰富的软骨基质。因此，滑膜和骨髓来源的MSC比脂肪和肌肉来源的MSC具有更大的体内软骨形成潜力，但滑膜来源的MSC具有增殖潜力更大的优势。需要更高的细胞密度和骨膜补片才能使滑膜来源的MSC产生大量软骨基质。
