Bernardo M E, Emons J A M, Karperien M, Nauta A J, Willemze R, Roelofs H, Romeo S, Marchini A, Rappold G A, Vukicevic S, Locatelli F, Fibbe W E
Pediatric Hematology/Oncology, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy.
Connect Tissue Res. 2007;48(3):132-40. doi: 10.1080/03008200701228464.
Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiation into several mesodermal lineages. These cells have been isolated from various tissues, such as adult bone marrow, placenta, and fetal tissues. The comparative potential of these cells originating from different tissues to differentiate into the chondrogenic lineage is still not fully defined. The aim of our study was to investigate the chondrogenic potential of MSCs isolated from different sources. MSCs from fetal and adult tissues were phenotypically characterized and examined for their differentiation capacity, based on morphological criteria and expression of extracellular matrix components. Our results show that both fetal and adult MSCs have chondrogenic potential under appropriate conditions. The capacity of bone marrow-derived MSCs to differentiate into chondrocytes was reduced on passaging of cells. MSCs of bone marrow origin, either fetal or adult, exhibit a better chondrogenesis than fetal lung- and placenta-derived MSCs, as demonstrated by the appearance of typical morphological features of cartilage, the intensity of toluidine blue staining, and the expression of collagen type II, IX, and X after culture under chondrogenic conditions. As MSCs represent an attractive tool for cartilage tissue repair strategies, our data suggest that bone marrow should be considered the preferred MSC source for these therapeutic approaches.
间充质干细胞（MSCs）是能够分化为多种中胚层谱系的多能细胞。这些细胞已从各种组织中分离出来，如成人骨髓、胎盘和胎儿组织。源自不同组织的这些细胞分化为软骨谱系的相对潜力仍未完全明确。我们研究的目的是调查从不同来源分离的间充质干细胞的软骨形成潜力。基于形态学标准和细胞外基质成分的表达，对来自胎儿和成人组织的间充质干细胞进行表型特征分析并检测其分化能力。我们的结果表明，在适当条件下，胎儿和成人的间充质干细胞都具有软骨形成潜力。细胞传代后，骨髓来源的间充质干细胞分化为软骨细胞的能力降低。在软骨形成条件下培养后，通过软骨典型形态特征的出现、甲苯胺蓝染色强度以及II型、IX型和X型胶原蛋白的表达证明，无论是胎儿还是成人的骨髓来源间充质干细胞都比胎儿肺和胎盘来源的间充质干细胞表现出更好的软骨形成能力。由于间充质干细胞是软骨组织修复策略的有吸引力的工具，我们的数据表明，骨髓应被视为这些治疗方法的首选间充质干细胞来源。
