Ma Ting, Huang Zhinong, Ren Pei-Gen, McCally Ryan, Lindsey Derek, Smith R L, Goodman Stuart B
Department of Orthopaedic Surgery, Stanford University, School of Medicine, Stanford, CA, USA.
Bone and Joint Rehabilitation R&D Center, The Palo Alto Veterans Administration Health Care Center, Palo Alto, CA, USA.
Biomaterials. 2008 Sep;29(27):3738-3742. doi: 10.1016/j.biomaterials.2008.05.031. Epub 2008 Jun 18.
Wear debris affects both initial osseointegration and subsequent bone remodeling of total joint replacements (TJRs). To study the complex cascade associated with the continuous generation of particles, a robust animal model is essential. To date, an animal model that incorporates continuously delivered particles to an intramedullary orthopaedic implant has not been available. In this study, we successfully infused clinically relevant ultra high molecular weight polyethylene particles, previously isolated from joint simulator tests, to the intramedullary space of the mouse femur for 4 weeks using a subcutaneous osmotic pump. Reduction of bone volume following the 4-week infusion of UHMWPE was detected by microCT. UHMWPE particles also changed the level of Alkaline Phosphatase expression in the infused femurs. Continuous infusion of particles to the murine bone-implant interface simulated the clinical scenario of local polymer wear particle generation and delivery in humans and can be used to further study the biological processes associated with wear debris particles.
磨损颗粒会影响全关节置换术（TJRs）的初始骨整合及随后的骨重塑。为研究与颗粒持续产生相关的复杂级联反应，一个可靠的动物模型至关重要。迄今为止，尚无一种将持续递送颗粒整合到髓内骨科植入物的动物模型。在本研究中，我们使用皮下渗透泵成功地将先前从关节模拟器试验中分离出的具有临床相关性的超高分子量聚乙烯颗粒输注到小鼠股骨的髓腔内，持续4周。通过显微CT检测到在输注超高分子量聚乙烯4周后骨体积减少。超高分子量聚乙烯颗粒还改变了输注股骨中碱性磷酸酶的表达水平。向小鼠骨-植入物界面持续输注颗粒模拟了人类局部聚合物磨损颗粒产生和递送的临床情况，可用于进一步研究与磨损碎屑颗粒相关的生物学过程。
