Albrecht S, From L, Kahn H J
Department of Pathology, Women's College Hospital, University of Toronto, Ontario, Canada.
J Cutan Pathol. 1991 Apr;18(2):75-80. doi: 10.1111/j.1600-0560.1991.tb00131.x.
Staining of elastic fibres with antilysozyme antibodies has been noted previously. In this study, we examined the staining pattern of dermal elastic fibres in aging, solar elastosis, and lesional skin of pseudoxanthoma elasticum (PXE) using an antibody to lysozyme and the indirect-peroxidase technique. To assess the effects of aging, sun-protected skin (buttock) from a younger and an older group of patients was used. Sun damage was studied in skin specimens from varying sun-exposed body regions (trunk; head and neck). No staining was seen in sun-protected skin from younger individuals, whereas sun-protected skin from older persons had scattered positive fibres. Solar elastotic material was intensely positive and the number of positive fibres appeared to correlate with the amount of sun damage. Abnormal elastic fibres in PXE also stained positively, but less intensely, than fibres in solar elastosis. This study shows that changes in the elastic fibres due to degenerative processes or genetic factors results in altered antigenic expression of the fibres. This may be an epiphenomenon secondary to changes in proteoglycans, which are known to occur with solar elastosis and PXE, or may represent an adaptive phenomenon to maintain the elastic properties of the altered fibres or to decrease their antigenicity.
此前已有用抗溶菌酶抗体对弹性纤维进行染色的相关报道。在本研究中，我们使用抗溶菌酶抗体和间接过氧化物酶技术，检测了衰老、日光性弹力纤维变性以及弹性假黄瘤（PXE）皮损处皮肤中真皮弹性纤维的染色模式。为评估衰老的影响，我们选取了年轻组和老年组患者的防晒皮肤（臀部）。对来自不同日光暴露身体部位（躯干、头颈部）的皮肤标本进行日光损伤研究。年轻个体的防晒皮肤未见染色，而老年个体的防晒皮肤有散在的阳性纤维。日光性弹力纤维变性物质呈强阳性，阳性纤维数量似乎与日光损伤程度相关。PXE中的异常弹性纤维也呈阳性染色，但强度低于日光性弹力纤维变性中的纤维。本研究表明，由退行性过程或遗传因素导致的弹性纤维变化会引起纤维抗原性表达改变。这可能是继发于蛋白聚糖变化的一种附带现象，已知蛋白聚糖变化会发生在日光性弹力纤维变性和PXE中，或者可能代表一种适应性现象，以维持改变后纤维的弹性特性或降低其抗原性。
