Polyak Maximilian M R, Grosche Astrid, Towl Simon, Morton Alison J
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, The Transplant Center at Shands Hospital, University of Florida, Gainesville, FL 32610, USA.
Vet Surg. 2008 Jun;37(4):383-9. doi: 10.1111/j.1532-950X.2008.00392.x.
To evaluate the influence of a kidney perfusion solution on early graft function in dogs.
Experimental, randomized study.
Intact adult male mongrel dogs (n=12).
Dogs had renal autograft transplantation without ureteroneocystotomy with contralateral nephrectomy. Kidney graft flushing with a novel organ perfusion solution was compared with flushing with saline (0.9% NaCl) solution. Serum creatinine (Cr) and blood urea nitrogen (BUN) concentrations were measured daily posttransplant for 7 days. Ultrasound-guided renal biopsy was performed on postoperative day 1 for electron microscopic evaluation. Dogs were euthanatized on day 7.
All dogs completed the study. Cr and BUN concentrations of the saline group were significantly greater than the organ perfusion solution group on each postoperative day (P=.01 for S Cr; P=.001 for BUN). Electron micrographs of nuclei and mitochondria from convoluted proximal tubule cells indicated profound ultrastructural disruptions in the saline group and mild ultrastructural disruptions in the organ perfusion solution group.
Flushing solution composition can influence early graft function in live donor kidney transplantation.
Use of a specialized flushing solution can improve early graft function in canine kidney transplantation, independent of antigen-mediated events.
评估一种肾脏灌注液对犬早期移植肾功能的影响。
实验性随机研究。
成年雄性杂种犬（n = 12）。
犬行自体肾移植，不做输尿管膀胱吻合术，对侧肾切除。将用一种新型器官灌注液冲洗肾移植与用生理盐水（0.9%氯化钠）溶液冲洗进行比较。移植后7天每天测定血清肌酐（Cr）和血尿素氮（BUN）浓度。术后第1天进行超声引导下肾活检以进行电子显微镜评估。犬在第7天安乐死。
所有犬均完成研究。术后各天，生理盐水组的Cr和BUN浓度均显著高于器官灌注液组（血清Cr，P = 0.01；BUN，P = 0.001）。曲部近端小管细胞核和线粒体的电子显微镜照片显示，生理盐水组有严重的超微结构破坏，而器官灌注液组有轻度的超微结构破坏。
冲洗液成分可影响活体供肾移植的早期移植肾功能。
使用专门的冲洗液可改善犬肾移植的早期移植肾功能，与抗原介导的事件无关。
