Li Xin, Quan Chun-Shan, Yu Hui-Ying, Fan Sheng-Di
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
FEMS Microbiol Lett. 2008 Aug;285(2):250-6. doi: 10.1111/j.1574-6968.2008.01238.x. Epub 2008 Jun 28.
A novel compound (named CF66I) produced by Burkholeria cepacia CF-66 strain was investigated for its antifungal activity against Candida albicans. This compound exhibited excellent antifungal activity in a dose- and time-dependent manner. Uptake analysis revealed that the compound preferentially acted against the fungal cell wall, and was also able to enter the cells. Transmission electron microscopy indicated that this compound caused loosening of the cell wall and a significant increase in the cell wall thickness was noted; however, no alterations were observed in the contents of the cell wall components. CF66I probably affected the normal assembly and integration of fungal cell wall components by interrupting the weak interactions between them, such as hydrogen and hydrophobic bonds. Propidium iodide (PI) staining indicated that on exposure to CF66I C. albicans cells became permeable to PI. Marked alterations in lipid and sterol contents were observed, and the major changes were a depletion of total lipids and ergosterol, concomitant with an increase in lanosterol content. These observations suggested that the novel compound CF66I may have considerable potential for development of a new class of antifungal agents.
对洋葱伯克霍尔德菌CF - 66菌株产生的一种新型化合物（命名为CF66I）针对白色念珠菌的抗真菌活性进行了研究。该化合物以剂量和时间依赖性方式表现出优异的抗真菌活性。摄取分析表明，该化合物优先作用于真菌细胞壁，并且还能够进入细胞。透射电子显微镜显示该化合物导致细胞壁松弛，并且观察到细胞壁厚度显著增加；然而，未观察到细胞壁成分含量的改变。CF66I可能通过中断真菌细胞壁成分之间的弱相互作用（如氢键和疏水键）来影响其正常组装和整合。碘化丙啶（PI）染色表明，暴露于CF66I时，白色念珠菌细胞对PI变得可渗透。观察到脂质和甾醇含量有明显变化，主要变化是总脂质和麦角甾醇减少，同时羊毛甾醇含量增加。这些观察结果表明，新型化合物CF66I在开发新型抗真菌剂方面可能具有相当大的潜力。
