Nakano K, Miyamoto E, Tamura K, Nemoto H, Fujita K, Nomura R, Ooshima T
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan.
Oral Dis. 2008 Oct;14(7):658-64. doi: 10.1111/j.1601-0825.2008.01452.x. Epub 2008 Jun 19.
There is scant information available regarding the distribution of periodontal bacterial species in children and adolescents over an extended period. The purpose of this study was to compare bacterial profiles in the same individuals over a period of 7 years.
Twenty-six children and adolescents from whom dental plaque and saliva specimens were obtained during both the first (1999-2000) and second (2006-2007) periods, were analyzed. Bacterial DNA was extracted from each specimen and the presence of 10 periodontal bacterial species was determined using a PCR method, with a focus on the red complex species of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia.
Subjects with red complex species in saliva specimens obtained during the second collection possessed a significantly higher number of total bacterial species than those without. The detection rate of the red complex species in the second collection period samples was significantly greater in subjects who had two or more species detected in samples taken during the first collection compared with the other subjects.
Subjects possessing red complex species may be at possible risk for infection with a high number of periodontal bacterial species during adolescent and younger adult years.
关于儿童和青少年牙周细菌种类在较长时期内的分布情况，现有信息匮乏。本研究的目的是比较同一人群在7年时间内的细菌谱。
对26名儿童和青少年进行分析，在第一个时期（1999 - 2000年）和第二个时期（2006 - 2007年）均采集了他们的牙菌斑和唾液样本。从每个样本中提取细菌DNA，并使用聚合酶链反应（PCR）方法测定10种牙周细菌种类的存在情况，重点关注牙龈卟啉单胞菌、具核梭杆菌和福赛坦氏菌这三种红色复合体细菌。
在第二次采集的唾液样本中含有红色复合体细菌的受试者所拥有的细菌种类总数显著高于不含该细菌的受试者。与其他受试者相比，在第一次采集样本时检测出两种或更多种细菌的受试者在第二次采集时期样本中红色复合体细菌的检出率显著更高。
携带红色复合体细菌的受试者在青少年和青年时期可能面临感染大量牙周细菌种类的风险。
