Wu C H, Nomura K, Goldammer T, Hadfield T, Dalrymple B P, McWilliam S, Maddox J F, Womack J E, Cockett N E
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322-4700, USA.
Anim Genet. 2008 Oct;39(5):459-67. doi: 10.1111/j.1365-2052.2008.01751.x. Epub 2008 Jun 28.
In this study, we constructed high-resolution radiation hybrid (RH) and comparative maps of ovine chromosomes or chromosomal segments that are homologous to human chromosome 6 (HSA6). A total of 251 markers were successfully genotyped across the recently developed USUoRH5000 whole-genome panel; 208 of these markers were assigned to five RH linkage groups distributed on three ovine chromosomes (OAR8, 9 and 20). The RH maps have good correspondence with previous chromosome painting data, although a small centromeric region on OAR9 that is homologous to HSA6 had not been previously detected using human chromosome paints on ovine chromosomal spreads. High percentages of the ovine markers were identified as orthologues in the bovine (86.3%), dog (85.8%), horse (69.3%) and human (88.7%) genomes. These maps contribute to investigations in mammalian chromosome evolution and the search for economic trait loci in sheep.
在本研究中，我们构建了与人类6号染色体（HSA6）同源的绵羊染色体或染色体片段的高分辨率辐射杂种（RH）图谱和比较图谱。在最近开发的USUoRH5000全基因组面板上，总共成功对251个标记进行了基因分型；其中208个标记被分配到分布在三条绵羊染色体（OAR8、9和20）上的五个RH连锁群中。尽管使用人类染色体对绵羊染色体涂片进行染色时，先前未检测到OAR9上与HSA6同源的一个小着丝粒区域，但RH图谱与先前的染色体涂染数据具有良好的对应关系。在牛（86.3%）、狗（85.8%）、马（69.3%）和人类（88.7%）基因组中，高比例的绵羊标记被鉴定为直系同源物。这些图谱有助于哺乳动物染色体进化的研究以及绵羊经济性状基因座的寻找。
