Kim Hyun-Chul, Lee Soo-Keun, Jeon Won Bae, Lyu Hong-Kun, Lee Seung Woo, Jeong Sang Won
Division of Nano-Bio Technology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 704-230, Republic of Korea.
Ultramicroscopy. 2008 Sep;108(10):1379-83. doi: 10.1016/j.ultramic.2008.04.052. Epub 2008 May 10.
The preparation of a new poly(thiophene) with pendant N-hydroxysuccinimide ester groups and its application to immobilization of biomolecules are reported. A thiophene derivative of N-hydroxysuccinimide ester was polymerized with FeCl3 in chloroform and the resulting poly(thiophene) was characterized by nuclear magnetic resonance (NMR), Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). This polymer reacts with amine-bearing molecules to yield new poly(thiophene) derivatives and the specific interactions at the side groups could be detected. Thus, a self-assembled monolayer (SAM) using the polymer was formed on a gold-coated quartz cell and anti-C-reactive protein (anti-CRP) was immobilized. The binding behavior of CRP on the surface was monitored by use of a surface plasmon resonance (SPR) sensor system.
