Li Y R, Li Q, Yang J M, Zhou X M, Yin X M, Zhao D M
National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
Mol Cell Probes. 2008 Aug;22(4):255-8. doi: 10.1016/j.mcp.2008.04.004. Epub 2008 May 10.
Doppel (prion-like protein, Dpl) may act as a useful molecular marker in tumor diagnosis and in tumor grade definition, as over-expression of Dpl protein has been found in tumors with different histologic origin. Accordingly, the quantitative analysis of the expression of Dpl in different tissues is essential for understanding its role in tumor progression and cancer diagnostic. Herein we report Dpl mRNA quantification in golden hamster by calibrated highly sensitive externally standardized real-time RT-PCR with LightCycler instrument. Total RNA was isolated from nine different organs of golden hamster in different stages of development: from neonatal to adult golden hamster. Highest level of Dpl mRNA was detected in the testis, and lower levels of Dpl mRNA were detected in the following tissues: spleen, heart, bone marrow, skeletal muscles and neocortex (only in neonatal hamster). The expression of Dpl was not detected in kidney, liver and lung. This is the first study to report the expression of Dpl in bone marrow of murine and the difference of expression levels of Dpl in testis between adult and neonatal hamsters.
多普蛋白（朊病毒样蛋白，Dpl）可能是肿瘤诊断和肿瘤分级定义中一种有用的分子标志物，因为在不同组织学起源的肿瘤中已发现Dpl蛋白过表达。因此，对不同组织中Dpl表达进行定量分析对于理解其在肿瘤进展和癌症诊断中的作用至关重要。在此，我们报告使用LightCycler仪器通过校准的高灵敏度外部标准化实时逆转录聚合酶链反应对金黄地鼠中的Dpl信使核糖核酸进行定量分析。从处于不同发育阶段（从新生到成年）的金黄地鼠的九个不同器官中分离出总核糖核酸。在睾丸中检测到最高水平的Dpl信使核糖核酸，在以下组织中检测到较低水平的Dpl信使核糖核酸：脾脏、心脏、骨髓、骨骼肌和新皮质（仅在新生地鼠中）。在肾脏、肝脏和肺中未检测到Dpl的表达。这是第一项报告Dpl在鼠骨髓中的表达以及成年和新生地鼠睾丸中Dpl表达水平差异的研究。
