Lee H C, Berka K M, Folk N L, Pagliaro E M, Carroll-Reho J, Brubaker T L, Gaensslen R E
Connecticut State Police Forensic Science Laboratory, Meriden.
J Forensic Sci. 1991 May;36(3):639-55.
A combination absorption-elution, two-dimensional absorption-inhibition procedure was used to determine the ABH antigen composition of a series of human bone specimens of known ABO type that had been aged up to nine months under dry and humid conditions at ambient temperature, 37 degrees C, and 56 degrees C; at ambient temperature in dry and wet soil; and buried in soil outdoors. Grouping data for the separate elution and inhibition testing, as well as for the combination procedure, are given. The combination method was found to be a highly reliable procedure for bone tissue ABH typing. Some data on microbial contaminants of human bone specimens aging in soil, and their effects on ABH typing results, are presented. No direct correlation between the properties of microbial contaminants and specific changes in the ABH antigenic composition of aging bone tissue specimens could be ascertained. Data on IGH antigen determination and on the quantitation of immunoglobulin G (IgG) in human bone tissue extracts indicated that immunoglobulin levels were typically too low to expect routinely successful Gm antigen testing results. However, these factors can sometimes be determined in fresh bone tissue extracts, particularly if the extracts are concentrated.
采用吸收-洗脱、二维吸收-抑制联合方法，对一系列已知ABO血型的人类骨标本的ABH抗原组成进行测定。这些标本在环境温度、37℃和56℃的干燥及潮湿条件下放置长达9个月；在环境温度下置于干土和湿土中；以及埋于户外土壤中。给出了单独洗脱和抑制试验以及联合方法的分组数据。结果发现联合方法是骨组织ABH分型的一种高度可靠的方法。文中还给出了人类骨标本在土壤中老化时的微生物污染物及其对ABH分型结果影响的一些数据。未确定微生物污染物的特性与老化骨组织标本ABH抗原组成的特定变化之间存在直接相关性。关于人类骨组织提取物中IGH抗原测定和免疫球蛋白G（IgG）定量的数据表明，免疫球蛋白水平通常过低，难以期望常规获得成功的Gm抗原检测结果。然而，这些因素有时可以在新鲜骨组织提取物中测定，特别是如果提取物经过浓缩。
