Rodrigues L E, Gaudenzi T F
Rev Bras Pesqui Med Biol. 1976 Sep;9(4):187-91.
Modifications of the Warburg's flask were suggested for the study of the electric current effect upon enzymatic systems under directly stimulation "in vitro" and "in vivo" conditions. The proposed modifications made on the Warburg's flask were basically constituted by the insertion of two platinum and mercury electrodes in the principal reservoir. In order to demonstrate the efficiency and viability of the suggested modification, one experience concerning the effect of the electric current upon the cerebral glutamate decarboxylase activity was shown. The apoenzyme substrate system was activated 1.81 times by the electrical stimulation in absence of the coenzyme. In a similar way the complete system, that is, the apoenzyme-coenzyme-substrate was also stimulated. The physical phenomena seem to be of importance for the understanding of the physiology and biochemistry of the nervous tissue.
为了研究在“体外”和“体内”直接刺激条件下电流对酶系统的影响，有人提出了对瓦氏呼吸计的改进方法。对瓦氏呼吸计所做的改进主要是在主容器中插入两个铂电极和汞电极。为了证明所建议改进方法的有效性和可行性，展示了一个关于电流对脑谷氨酸脱羧酶活性影响的实验。在没有辅酶的情况下，通过电刺激，脱辅酶底物系统的活性提高了1.81倍。同样，完整的系统，即脱辅酶-辅酶-底物系统也受到了刺激。物理现象似乎对理解神经组织的生理学和生物化学很重要。
