Matsuda M, Tsukada N, Miyagi K, Yanagisawa N
Department of Neurology, Shinshu University, Matsumoto, Japan.
J Neurol Sci. 1991 Mar;102(1):100-4. doi: 10.1016/0022-510x(91)90099-s.
The production of interleukin-1 (IL-1) by peripheral blood mononuclear cells (MNC) was assessed in patients with relapsing multiple sclerosis (MS) in both the active and inactive phase, in chronic progressive MS patients, in other neurological diseases, and in healthy subjects. Production was determined by measuring the IL-1 concentration in cultures with MNC supernatants using enzyme-linked immunosorbent assay (ELISA). IL-1 in sera of MS patients and healthy subjects also was investigated. MNC IL-1 alpha production was significantly higher in MS patients (180.2 +/- 177.5 pg/ml) than in healthy subjects (66.2 +/- 66.0 pg/ml) (P less than 0.05). Relapsing MS patients in the active phase had significantly higher MNC IL-1 alpha concentrations (360.1 +/- 130.0 pg/ml) than normal subjects (P less than 0.001), but MNC IL-1 alpha production in patients with relapsing MS in the inactive phase (65.3 +/- 52.8 pg/ml) or chronic progressive MS (80.9 +/- 71.9 pg/ml) was not increased significantly. MNC IL-1 beta production in MS patients was not elevated significantly. IL-1 alpha and -1 beta were not detected in sera of MS patients. The correlation between increased IL-1 alpha production and the clinical course of MS suggests that activated MNC may play a role in the pathogenesis of MS.
对复发型多发性硬化症（MS）患者在活动期和非活动期、慢性进展型MS患者、其他神经系统疾病患者以及健康受试者外周血单个核细胞（MNC）白细胞介素-1（IL-1）的产生情况进行了评估。通过酶联免疫吸附测定法（ELISA）测量MNC上清液培养物中的IL-1浓度来确定其产生量。还对MS患者和健康受试者血清中的IL-1进行了研究。MS患者MNC的IL-1α产生量（180.2±177.5 pg/ml）显著高于健康受试者（66.2±66.0 pg/ml）（P<0.05）。复发型MS活动期患者的MNC的IL-1α浓度（360.1±130.0 pg/ml）显著高于正常受试者（P<0.001），但复发型MS非活动期患者（65.3±52.8 pg/ml）或慢性进展型MS患者（80.9±71.9 pg/ml）的MNC的IL-1α产生量未显著增加。MS患者MNC的IL-1β产生量未显著升高。在MS患者血清中未检测到IL-1α和-1β。IL-1α产生量增加与MS临床病程之间的相关性表明，活化的MNC可能在MS发病机制中起作用。
