多发性硬化症患者长期培养的脑脊液淋巴细胞中染色体畸变频率增加。

Increased frequency of chromosome aberrations in long-term cultured cerebrospinal fluid lymphocytes of patients with multiple sclerosis.

作者信息

Sandberg-Wollheim M, Kristoffersson U, Mandahl N, Högstedt B

机构信息

Department of Neurology, University Hospital, Lund, Sweden.

出版信息

J Neurol Sci. 1991 Mar;102(1):46-50. doi: 10.1016/0022-510x(91)90092-l.

DOI:10.1016/0022-510x(91)90092-l

PMID:1856732

Abstract

A method employing long-term lymphocyte culturing was developed to study chromosome aberrations in samples with very few cells. It was used to examine lymphocytes from the cerebrospinal fluid (CSF) and peripheral blood (PB) in 23 patients with clinically definite multiple sclerosis (MS), nine patients with other neurological diseases (OND), and eight healthy individuals. MS patients had significantly more aberrations in CSF lymphocytes than in PB lymphocytes (6.4 vs 4.1; P = 0.003). In contrast, no such difference was noted among patients with OND (3.8 vs. 3.7; P = 0.89) or healthy controls (3.6 vs 3.5; P = 0.90). CSF lymphocytes from MS patients had more aberrations than CSF lymphocytes from healthy controls (P = 0.012), but there was no difference between PB lymphocytes from MS patients and controls (P = 0.58). The patients with OND were similar to healthy controls both in CSF (3.8 vs 3.6; P = 0.91) and PB lymphocytes (3.7 vs 3.5; P = 0.90).

摘要

开发了一种采用长期淋巴细胞培养的方法，用于研究细胞数量极少的样本中的染色体畸变。该方法用于检测23例临床确诊的多发性硬化症（MS）患者、9例患有其他神经系统疾病（OND）的患者以及8名健康个体的脑脊液（CSF）和外周血（PB）中的淋巴细胞。MS患者脑脊液淋巴细胞中的畸变明显多于外周血淋巴细胞（6.4对4.1；P = 0.003）。相比之下，OND患者（3.8对3.7；P = 0.89）或健康对照者（3.6对3.5；P = 0.90）之间未观察到这种差异。MS患者的脑脊液淋巴细胞畸变多于健康对照者的脑脊液淋巴细胞（P = 0.012），但MS患者的外周血淋巴细胞与对照者之间无差异（P = 0.58）。OND患者在脑脊液（3.8对3.6；P = 0.91）和外周血淋巴细胞（3.7对3.5；P = 0.90）方面均与健康对照者相似。